About: Study of phospholipid signalling pathway using fluorescently labelled substrates

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About: Study of phospholipid signalling pathway using fluorescently labelled substrates - sborník Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Phospholipid signalling pathway is one of the basic signalling mechanism in animal as well as plant cells. Phospholipases and phospholipids are the key components of this system. Phospholipases are divided based on the site of cleavage of the phospholipid molecules to phospholipase A2, C and D. The aim of this study was to established sophisticated method which let us to analyse products of signalling phospholipases in its entirety with one method. Phospholipid signalling pathway was studied insitu in tobacco cell suspension culture BY-2 using different types of fluorescently labelled (BODIPY) phosphatidylcholine. Incorporation of the fluorescent substrates to tobacco cell membranes was monitored by fluorescent microscopy and confirmed by pla smolysis. Fluorescent products of cleaved phosphatidylcholine were analysed by HP-TLC and newly developed HPLC methods. First the fluorescent standards of individual phospholipase products were prepared using commercial phospholipases and then used (en) Phospholipid signalling pathway is one of the basic signalling mechanism in animal as well as plant cells. Phospholipases and phospholipids are the key components of this system. Phospholipases are divided based on the site of cleavage of the phospholipid molecules to phospholipase A2, C and D. The aim of this study was to established sophisticated method which let us to analyse products of signalling phospholipases in its entirety with one method. Phospholipid signalling pathway was studied insitu in tobacco cell suspension culture BY-2 using different types of fluorescently labelled (BODIPY) phosphatidylcholine. Incorporation of the fluorescent substrates to tobacco cell membranes was monitored by fluorescent microscopy and confirmed by pla smolysis. Fluorescent products of cleaved phosphatidylcholine were analysed by HP-TLC and newly developed HPLC methods. First the fluorescent standards of individual phospholipase products were prepared using commercial phospholipases and then used
Title	Study of phospholipid signalling pathway using fluorescently labelled substrates - sborník Study of phospholipid signalling pathway using fluorescently labelled substrates - sborník (en)
skos:prefLabel	Study of phospholipid signalling pathway using fluorescently labelled substrates - sborník Study of phospholipid signalling pathway using fluorescently labelled substrates - sborník (en)
skos:notation	RIV/60461373:22330/03:00008962!RIV/2004/GA0/223304/N
http://linked.open.../vavai/riv/strany	99
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GA522/03/0353)
http://linked.open...vai/riv/dodaniDat	2004
http://linked.open...aciTvurceVysledku	Pejchar, Přemysl Valentová, Olga
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoká škola chemicko-technologická v Praze / Fakulta potravinářské a biochemické technologie
http://linked.open...dnocenehoVysledku	629576
http://linked.open...ai/riv/idVysledku	RIV/60461373:22330/03:00008962
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	phospholipid signalling pathway; fluorescently labelled substrates; tobacco cell suspension (en)
http://linked.open.../riv/klicoveSlovo	tobacco cell suspension phospholipid signalling pathway fluorescently labelled substrates
http://linked.open...ontrolniKodProRIV	[5981FE617202]
http://linked.open...v/mistoKonaniAkce	Santa Mario Imbaro
http://linked.open...i/riv/mistoVydani	Itálie
http://linked.open...i/riv/nazevZdroje	FEBS Advanced Course: Lipid signalling and membrane traffic
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	Defence mechanisms in rape seed (Brassica napus) participating in plant-pathogen interaction and induced resistance
http://linked.open...UplatneniVysledku	2003
http://linked.open...iv/tvurceVysledku	Martinec, Jan Pejchar, Přemysl Valentová, Olga
http://linked.open...vavai/riv/typAkce	WRD - Světová
http://linked.open.../riv/zahajeniAkce	2003-06-20 (xsd:date)
number of pages	1 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Advanced FEBS Course
http://localhost/t...ganizacniJednotka	22330

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