About: Regulation of plant membrane associated PIP2 - dependent phospholipase D activity by phosphorylation - sborník     Goto   Sponge   NotDistinct   Permalink

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  • Phospholipase D forms the major family of phospholipases that was first discovered and cloned in plants. The distinct biochemical properties of various PLDs might predetermine their different regulation and functions. Several factors have been reported to stimulate plant PLD activity including Ca2+, polyphosphoinositides, G-proteins, N-acylethanolamines, free fatty acids and membrane lipids but nothing is known about the regulatory role of protein modification. In this contribution we would like to present, that covalent modification by phosphorylation could be one of the regulation mechanisms of PLD activity in plants. C2 PIP2-dependent PLD(s) from 5 day hypocotyls of Brassica oleracea var. capitata associated with plasma membrane is probably phosphorylated. Preincubation of plasma membrane fraction with acid phosphatase resulted in concentration´-dependent inhibition of PIP2-dependent PLD activity. Furthermore, we have identified 5 peptide fragments in plasma membrane fraction, which masses corres (en)
  • Phospholipase D forms the major family of phospholipases that was first discovered and cloned in plants. The distinct biochemical properties of various PLDs might predetermine their different regulation and functions. Several factors have been reported to stimulate plant PLD activity including Ca2+, polyphosphoinositides, G-proteins, N-acylethanolamines, free fatty acids and membrane lipids but nothing is known about the regulatory role of protein modification. In this contribution we would like to present, that covalent modification by phosphorylation could be one of the regulation mechanisms of PLD activity in plants. C2 PIP2-dependent PLD(s) from 5 day hypocotyls of Brassica oleracea var. capitata associated with plasma membrane is probably phosphorylated. Preincubation of plasma membrane fraction with acid phosphatase resulted in concentration´-dependent inhibition of PIP2-dependent PLD activity. Furthermore, we have identified 5 peptide fragments in plasma membrane fraction, which masses corres
Title
  • Regulation of plant membrane associated PIP2 - dependent phospholipase D activity by phosphorylation - sborník
  • Regulation of plant membrane associated PIP2 - dependent phospholipase D activity by phosphorylation - sborník (en)
skos:prefLabel
  • Regulation of plant membrane associated PIP2 - dependent phospholipase D activity by phosphorylation - sborník
  • Regulation of plant membrane associated PIP2 - dependent phospholipase D activity by phosphorylation - sborník (en)
skos:notation
  • RIV/60461373:22330/03:00008483!RIV/2004/MSM/223304/N
http://linked.open.../vavai/riv/strany
  • 92
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(MSM 223300006)
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
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  • 625081
http://linked.open...ai/riv/idVysledku
  • RIV/60461373:22330/03:00008483
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • phospholipase D; phosphorylation; Brassica napus (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [014A50DAFA9C]
http://linked.open...v/mistoKonaniAkce
  • Germany
http://linked.open...i/riv/mistoVydani
  • Aachen
http://linked.open...i/riv/nazevZdroje
  • 1 st European Symposium on Plant Lipids
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http://linked.open...ichTvurcuVysledku
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http://linked.open...ocetUcastnikuAkce
http://linked.open...nichUcastnikuAkce
http://linked.open...UplatneniVysledku
http://linked.open...iv/tvurceVysledku
  • Hynek, Radovan
  • Potocký, Martin
  • Linek, Jan
  • Novotná, Zuzana
  • Valentová, Olga
http://linked.open...vavai/riv/typAkce
http://linked.open.../riv/zahajeniAkce
http://linked.open...n/vavai/riv/zamer
number of pages
http://purl.org/ne...btex#hasPublisher
  • RWTH Aachen
http://localhost/t...ganizacniJednotka
  • 22330
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