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  • In our work we screened several in vitro cultures of different plant species for their ability to transform PCBs and PAHs. The estimation of total extra- and intracellular peroxidase activity with and without xenobiotics was correlated with the ability to metabolise xenobiotics. Cultures with good transformation ability exhibited in the presence of xenobiotics higher level of peroxidases than the controls. We attempted to identify lignin peroxidase and manganese dependent peroxidase in plants, enzymes originally described in white rot fungi. These enzymes are responsible for the degradation of many environmental pollutants. The decolourisation of RBBR during the growth on agar plate was used as a rough screening method for plant cell cultures able to efficiently metabolise PCBs.
  • In our work we screened several in vitro cultures of different plant species for their ability to transform PCBs and PAHs. The estimation of total extra- and intracellular peroxidase activity with and without xenobiotics was correlated with the ability to metabolise xenobiotics. Cultures with good transformation ability exhibited in the presence of xenobiotics higher level of peroxidases than the controls. We attempted to identify lignin peroxidase and manganese dependent peroxidase in plants, enzymes originally described in white rot fungi. These enzymes are responsible for the degradation of many environmental pollutants. The decolourisation of RBBR during the growth on agar plate was used as a rough screening method for plant cell cultures able to efficiently metabolise PCBs. (en)
Title
  • Plant Enzymes in Metabolism of PCBs and PAHs
  • Plant Enzymes in Metabolism of PCBs and PAHs (en)
skos:prefLabel
  • Plant Enzymes in Metabolism of PCBs and PAHs
  • Plant Enzymes in Metabolism of PCBs and PAHs (en)
skos:notation
  • RIV/60461373:22330/01:00004568!RIV/2004/GA0/223304/N
http://linked.open.../vavai/riv/strany
  • 67
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  • P(GA203/99/1628)
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  • 691242
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  • RIV/60461373:22330/01:00004568
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  • Peroxidases, plants, polychlorinated biphenyls, polyaromatic hydrocarbons (en)
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  • [CB2B3284A8FF]
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  • Praha
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  • Neuveden
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  • Book of abstracts - Innovative approaches to the on-site asseeement remediation of contaminated sites.
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  • Macek, Tomáš
  • Macková, Martina
  • Chromá, Ludmila
  • Kučerová, Irena
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http://linked.open.../riv/zahajeniAkce
number of pages
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  • NATO Advanced Study, D. Reible (ed.)
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  • 22330
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