About: Fullerene C60 films of continuous and micropatterned morphology as substrates for adhesion and growth of bone cells

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Fullerenes C-60 were deposited on microscopic glass coverslips in the form of continuous layers (thickness 505 +/- 43 nm or 1090 +/- 8 nm) or micropatterned layers using a metallic mask with rectangular openings (128x98 mu m, spacing 50 mu m). Below the openings, the fullerenes formed bulges 484 +/- 5 nm or 1043 +/- 57 nm, in thickness. Very thin fullerene films were also found below the metallic bars of the grid. The adhesion and proliferation activities of human osteoblast-like MG 63 cells in cultures on the fullerene layers were similar as that on the control polystyrene dishes and glass coverslips. On the thick patterned layers. the cells grew preferentially in the grooves among the fullerene bulges. Although these grooves occupied only approximately 41% of the surface, they contained from 80% to 98% of the cells. Immunocytochemistry showed that the cells on all tested surfaces formed beta(1) integrin- and talin-containing focal adhesion plaques, a beta-actin cytoskeleton, and contained osteocalci Fullerenes C-60 were deposited on microscopic glass coverslips in the form of continuous layers (thickness 505 +/- 43 nm or 1090 +/- 8 nm) or micropatterned layers using a metallic mask with rectangular openings (128x98 mu m, spacing 50 mu m). Below the openings, the fullerenes formed bulges 484 +/- 5 nm or 1043 +/- 57 nm, in thickness. Very thin fullerene films were also found below the metallic bars of the grid. The adhesion and proliferation activities of human osteoblast-like MG 63 cells in cultures on the fullerene layers were similar as that on the control polystyrene dishes and glass coverslips. On the thick patterned layers. the cells grew preferentially in the grooves among the fullerene bulges. Although these grooves occupied only approximately 41% of the surface, they contained from 80% to 98% of the cells. Immunocytochemistry showed that the cells on all tested surfaces formed beta(1) integrin- and talin-containing focal adhesion plaques, a beta-actin cytoskeleton, and contained osteocalci (en)
Title	Fullerene C60 films of continuous and micropatterned morphology as substrates for adhesion and growth of bone cells Fullerene C60 films of continuous and micropatterned morphology as substrates for adhesion and growth of bone cells (en)
skos:prefLabel	Fullerene C60 films of continuous and micropatterned morphology as substrates for adhesion and growth of bone cells Fullerene C60 films of continuous and micropatterned morphology as substrates for adhesion and growth of bone cells (en)
skos:notation	RIV/60461373:22310/09:00021441!RIV10-MSM-22310___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA101/06/0226), P(GA204/06/0225), P(KAN400480701), P(LC06041), Z(AV0Z10100520), Z(AV0Z10480505), Z(AV0Z20430508), Z(AV0Z50110509), Z(MSM6046137302)
http://linked.open...iv/cisloPeriodika	2-3
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Švorčík, Václav Slepička, Petr
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoká škola chemicko-technologická v Praze / Fakulta chemické technologie
http://linked.open...dnocenehoVysledku	315687
http://linked.open...ai/riv/idVysledku	RIV/60461373:22310/09:00021441
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Nanoparticles; Biocompatibility; Surface structure; Biomedical application (en)
http://linked.open.../riv/klicoveSlovo	Nanoparticles Biocompatibility Surface structure Biomedical application
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[D2319E438502]
http://linked.open...i/riv/nazevZdroje	Diamond and Related Materials
http://linked.open...in/vavai/riv/obor	CD
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	NANOSTRUCTURES BASED ON CARBON AND POLYMERS FOR APPLICATION IN BIOELECTRONIC AND MEDICINE Preparation, modification and characterization of materials by energetic radiation. Adhesion, growth and differentiation of bone and vascular cells on carbon allotropes Surface-modified carbon composites as potential implants into human body
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	18
http://linked.open...iv/tvurceVysledku	Bačáková, Lucie Slepička, Petr Vacík, Jiří Švorčík, Václav Grausová, L. Vandrovcová, M. Bílková, P.
http://linked.open...ain/vavai/riv/wos	000264429300109
http://linked.open...n/vavai/riv/zamer	Specific effects in condensed systems with reduced dimension and broken symmetry Jaderná fyzika a příbuzné obory v základním, aplikovaném a interdisciplinárním výzkumu Physical and chemical processes in plasmas and theis applications Výzkum molekulárních a buněčných základů fyziologických a patofyziologických procesů s cílem objasnit mechanismy vzniku závažných onemocnění člověka Preparation and research of functional materials and material technologies using micro- and nanoscopic methods
issn	0925-9635
number of pages	9 (xsd:int)
http://localhost/t...ganizacniJednotka	22310
is http://linked.open...avai/riv/vysledek of	Fullerene C60 films of continuous and micropatterned morphology as substrates for adhesion and growth of bone cells Fullerene C60 films of continuous and micropatterned morphology as substrates for adhesion and growth of bone cells Fullerene C60 films of continuous and micropatterned morphology as substrates for adhesion and growth of bone cells Fullerene C60 films of continuous and micropatterned morphology as substrates for adhesion and growth of bone cells

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