About: DETECTION OF HOP STUNT VIROID WITHIN POLLEN GRAINS OF INFECTED PEACH TREES: EVIDENCE USING RT-PCR AND DOT BLOT HYBRIDIZATION

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Hop stunt viroid (HSVd) is the viroid with the broadest host range known so far, also including stone fruit trees. Until now, six viroids have been shown to be transmitted through seeds to seedlings, in one or more hosts, four of them have been detected in pollen, cucumber strain of HSVd being among them. High incidence of HSVd infections of peach trees was recently found in the Czech Republic indicating the possibility of spreading via pollen. That is why we tested the presence of HSVd in pollen grains . Closed flowers from peach trees of cultivars Sunhaven and NJC 103 known to be infected by HSVd were collected in March. Pollen grains were separated from air dried anthers and total RNA was extracted from 10 - 20 mg of pollen or intact anthers and from 100 mg of leaves or petals. PCR and molecular hybridization methods were used for HSVd detection. HSVd was consistently detected in pollen, anthers, petals as well as in leaves in each of at least three repetitions. This preliminary results have specia Hop stunt viroid (HSVd) is the viroid with the broadest host range known so far, also including stone fruit trees. Until now, six viroids have been shown to be transmitted through seeds to seedlings, in one or more hosts, four of them have been detected in pollen, cucumber strain of HSVd being among them. High incidence of HSVd infections of peach trees was recently found in the Czech Republic indicating the possibility of spreading via pollen. That is why we tested the presence of HSVd in pollen grains . Closed flowers from peach trees of cultivars Sunhaven and NJC 103 known to be infected by HSVd were collected in March. Pollen grains were separated from air dried anthers and total RNA was extracted from 10 - 20 mg of pollen or intact anthers and from 100 mg of leaves or petals. PCR and molecular hybridization methods were used for HSVd detection. HSVd was consistently detected in pollen, anthers, petals as well as in leaves in each of at least three repetitions. This preliminary results have specia (en)
Title	DETECTION OF HOP STUNT VIROID WITHIN POLLEN GRAINS OF INFECTED PEACH TREES: EVIDENCE USING RT-PCR AND DOT BLOT HYBRIDIZATION DETECTION OF HOP STUNT VIROID WITHIN POLLEN GRAINS OF INFECTED PEACH TREES: EVIDENCE USING RT-PCR AND DOT BLOT HYBRIDIZATION (en)
skos:prefLabel	DETECTION OF HOP STUNT VIROID WITHIN POLLEN GRAINS OF INFECTED PEACH TREES: EVIDENCE USING RT-PCR AND DOT BLOT HYBRIDIZATION DETECTION OF HOP STUNT VIROID WITHIN POLLEN GRAINS OF INFECTED PEACH TREES: EVIDENCE USING RT-PCR AND DOT BLOT HYBRIDIZATION (en)
skos:notation	RIV/60460709:_____/03:00005226!RIV/2004/MZE/410004/N
http://linked.open.../vavai/riv/strany	117
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(QC1359), Z(MSM 412100005)
http://linked.open...vai/riv/dodaniDat	2004
http://linked.open...aciTvurceVysledku	Hassan, Ahmed Ali Mohamed Ryšánek, Pavel
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Česká zemědělská univerzita v Praze / (nerozlišená součást)
http://linked.open...dnocenehoVysledku	603271
http://linked.open...ai/riv/idVysledku	RIV/60460709:_____/03:00005226
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	HSVd;PCR;dot blot hybridization;peach;pollen (en)
http://linked.open.../riv/klicoveSlovo	HSVd peach pollen dot blot hybridization PCR
http://linked.open...ontrolniKodProRIV	[646BABEA0ABE]
http://linked.open...v/mistoKonaniAkce	Valencia
http://linked.open...i/riv/mistoVydani	Valencia
http://linked.open...i/riv/nazevZdroje	Book of abstract 19th Int. Symp. Virus and Virus - like Diseases of Temperature Fruit Crops
http://linked.open...in/vavai/riv/obor	GF
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	Development of complex procedures of diagnostics of economically important and quarantine phytopatogenic organisms for developing of fruit trees certification system in the Czech Republic.
http://linked.open...UplatneniVysledku	2003
http://linked.open...iv/tvurceVysledku	Ryšánek, Pavel Hassan, Ahmed Ali Mohamed
http://linked.open...vavai/riv/typAkce	WRD - Světová
http://linked.open.../riv/zahajeniAkce	2003-07-21 (xsd:date)
http://linked.open...n/vavai/riv/zamer	http://linked.opendata.cz/resource/domain/vavai/zamer/MSM%20412100005
number of pages	1 (xsd:int)
http://purl.org/ne...btex#hasPublisher	ISHS

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