About: Identification of PCN species (Globodera rostochiensis, G. pallida) by using of ITS-1 region's polymorphism

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About: Identification of PCN species (Globodera rostochiensis, G. pallida) by using of ITS-1 region's polymorphism Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Actual methods of molecular analyses of DNA are routinely used at fields of zoological and botanical taxonomy, pest management and plant breeding. Knowledge of species-composition in populations of potato cyst nematodes (Globodera rostochiensis, G. pallida) is very important for selection of appropriate measure of regulation PCN's occurrence. The molecular method for distinguishing of PCN species is described at this article. There were used European populations of PCN - Šluknov (Ro1), Obersteinbach (Ro2), Harmerz (Ro5), Kalle (Pa2), Chavornay (Pa3), Delmsen (Pa3), and some cysts of unknown patotype from Kašperské hory (K) locality. Species-specific sets of primers for ITS-1 (Internal Transcribed Spacer 1) amplification were designed on base of known sequences ITS-1 of both PCN species by using of freeware Primers! for the World Wide Web. By using of set Fro1 - Rro1 was product 411bp detected (only in cause G. rostochiensis), by using of set Fpa2 - Rpa1 the product 239bp was detected (only G. pallida) Actual methods of molecular analyses of DNA are routinely used at fields of zoological and botanical taxonomy, pest management and plant breeding. Knowledge of species-composition in populations of potato cyst nematodes (Globodera rostochiensis, G. pallida) is very important for selection of appropriate measure of regulation PCN's occurrence. The molecular method for distinguishing of PCN species is described at this article. There were used European populations of PCN - Šluknov (Ro1), Obersteinbach (Ro2), Harmerz (Ro5), Kalle (Pa2), Chavornay (Pa3), Delmsen (Pa3), and some cysts of unknown patotype from Kašperské hory (K) locality. Species-specific sets of primers for ITS-1 (Internal Transcribed Spacer 1) amplification were designed on base of known sequences ITS-1 of both PCN species by using of freeware Primers! for the World Wide Web. By using of set Fro1 - Rro1 was product 411bp detected (only in cause G. rostochiensis), by using of set Fpa2 - Rpa1 the product 239bp was detected (only G. pallida) (en)
Title	Identification of PCN species (Globodera rostochiensis, G. pallida) by using of ITS-1 region's polymorphism Identification of PCN species (Globodera rostochiensis, G. pallida) by using of ITS-1 region's polymorphism (en)
skos:prefLabel	Identification of PCN species (Globodera rostochiensis, G. pallida) by using of ITS-1 region's polymorphism Identification of PCN species (Globodera rostochiensis, G. pallida) by using of ITS-1 region's polymorphism (en)
skos:notation	RIV/60460709:_____/02:00004302!RIV/2003/MZE/410003/N
http://linked.open.../vavai/riv/strany	486-489
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(EP9107), Z(MSM 412100002)
http://linked.open...iv/cisloPeriodika	11
http://linked.open...vai/riv/dodaniDat	2003
http://linked.open...aciTvurceVysledku	Skupinová, Sylva Vejl, Pavel Sedlák, Petr
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Česká zemědělská univerzita v Praze / (nerozlišená součást)
http://linked.open...dnocenehoVysledku	648291
http://linked.open...ai/riv/idVysledku	RIV/60460709:_____/02:00004302
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Globodera rostochiensis, Globodera pallida, ITS - 1 region, PCR, DNA markery, identifikace druhu (en)
http://linked.open.../riv/klicoveSlovo	PCR Globodera pallida identifikace druhu Globodera rostochiensis ITS DNA markery
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[F4DDBD9A06D0]
http://linked.open...i/riv/nazevZdroje	Rostlinná výroba (Plant Production)
http://linked.open...in/vavai/riv/obor	GE
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	Analysis of the Resistance Genes Donors against Potato Cyst Nematodes in Potato Genofond by DNA Markers
http://linked.open...UplatneniVysledku	2002
http://linked.open...v/svazekPeriodika	48
http://linked.open...iv/tvurceVysledku	Domkářová, J. Sedlák, Petr Vejl, Pavel Skupinová, Sylva
http://linked.open...n/vavai/riv/zamer	http://linked.opendata.cz/resource/domain/vavai/zamer/MSM%20412100002
issn	0370-663X
number of pages	4 (xsd:int)

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