About: Segregation of DNA markers of potato (Solanum tuberosum ssp. tuberosum L.) resistance against Ro1 patotype Globodera rostochiensis in selected F1 progeny

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About: Segregation of DNA markers of potato (Solanum tuberosum ssp. tuberosum L.) resistance against Ro1 patotype Globodera rostochiensis in selected F1 progeny Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Marker assisted selection is the fast and objective method for detection of resistance majorgenes. This method is so usable to identification of some candidate genes of quantitative resistance. Genetic markers based on Polymerase Chain Reaction (PCR) were used for evaluation of F1 progenies Ornella x Mira and Tábor x Mira. Cultivar Mira has declare resistance against Ro1 pathotype G. rostochiensis. Cultivars Ornela and Tábor are to Ro1 susceptible. There were used seedlings of F1 generations for analyses. Plants were cultivated in greenhouse. DNA was isolated from tissue discs by %22GenElute Plant Genomic DNA Kit%22 (Sigma, SRN). For bulked analyses was used PCR marker of majorgene H1 according to (Niewöhner et al., 1995). Size of this marker was 760bp. Standard infection tests with Ro1 pathotype G. rostochiensis according to Potoček et al. (1991) in all of the analysed genotypes were made as well. Segregation ratios of F1 progenies were determined. These ratios have described segregation of resistance ma Marker assisted selection is the fast and objective method for detection of resistance majorgenes. This method is so usable to identification of some candidate genes of quantitative resistance. Genetic markers based on Polymerase Chain Reaction (PCR) were used for evaluation of F1 progenies Ornella x Mira and Tábor x Mira. Cultivar Mira has declare resistance against Ro1 pathotype G. rostochiensis. Cultivars Ornela and Tábor are to Ro1 susceptible. There were used seedlings of F1 generations for analyses. Plants were cultivated in greenhouse. DNA was isolated from tissue discs by %22GenElute Plant Genomic DNA Kit%22 (Sigma, SRN). For bulked analyses was used PCR marker of majorgene H1 according to (Niewöhner et al., 1995). Size of this marker was 760bp. Standard infection tests with Ro1 pathotype G. rostochiensis according to Potoček et al. (1991) in all of the analysed genotypes were made as well. Segregation ratios of F1 progenies were determined. These ratios have described segregation of resistance ma (en)
Title	Segregation of DNA markers of potato (Solanum tuberosum ssp. tuberosum L.) resistance against Ro1 patotype Globodera rostochiensis in selected F1 progeny Segregation of DNA markers of potato (Solanum tuberosum ssp. tuberosum L.) resistance against Ro1 patotype Globodera rostochiensis in selected F1 progeny (en)
skos:prefLabel	Segregation of DNA markers of potato (Solanum tuberosum ssp. tuberosum L.) resistance against Ro1 patotype Globodera rostochiensis in selected F1 progeny Segregation of DNA markers of potato (Solanum tuberosum ssp. tuberosum L.) resistance against Ro1 patotype Globodera rostochiensis in selected F1 progeny (en)
skos:notation	RIV/60460709:_____/02:00004115!RIV/2003/MZE/410003/N
http://linked.open.../vavai/riv/strany	480-485
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(EP9107), Z(MSM 412100002)
http://linked.open...iv/cisloPeriodika	11
http://linked.open...vai/riv/dodaniDat	2003
http://linked.open...aciTvurceVysledku	Skupinová, Sylva Vejl, Pavel Sedlák, Petr
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Česká zemědělská univerzita v Praze / (nerozlišená součást)
http://linked.open...dnocenehoVysledku	663157
http://linked.open...ai/riv/idVysledku	RIV/60460709:_____/02:00004115
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	brambor, Solanum tuberosum ssp. tuberosum L., DNA markery, PCR, Ro1 patotyp Globodera rostochiensis, rezistence, majorgen H1, segregační analýza (en)
http://linked.open.../riv/klicoveSlovo	PCR brambor rezistence DNA markery Ro1 patotyp Globodera rostochiensis Solanum tuberosum ssp. tuberosum L. majorgen H1 segregační analýza
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[E9CD000B1DDA]
http://linked.open...i/riv/nazevZdroje	Rostlinná výroba (Plant Production)
http://linked.open...in/vavai/riv/obor	GE
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	Analysis of the Resistance Genes Donors against Potato Cyst Nematodes in Potato Genofond by DNA Markers
http://linked.open...UplatneniVysledku	2002
http://linked.open...v/svazekPeriodika	48
http://linked.open...iv/tvurceVysledku	Domkářová, J. Sedlák, Petr Vejl, Pavel Skupinová, Sylva
http://linked.open...n/vavai/riv/zamer	http://linked.opendata.cz/resource/domain/vavai/zamer/MSM%20412100002
issn	0370-663X
number of pages	5 (xsd:int)

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