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rdf:type
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Description
| - ITS úseky predstavujú zdroj variability rDNA oblastí využiteľný v hodnotení genetických zdrojov jakonu ako molekulárne markéry fylogenetických vzťahov. V práci bol vypracovaný protokol pre PCR identifikáciu úsekov medzi veľkou a malou podjednotkou rRNA pre následnú sekvenáciu pri trojici Bolívijských krajových odrôd jakonu (Smallanthus sonchifolius, Poepp. and Endl.). Identifikované úseky boli následne sekvenované a porovnané so sekvenciami ITS v databázach. Analyzované ITS úseky Bolívijských genotypov jakonu vykazujú variabilitu v pozícii nukleotidov 163-164 a 235-236 v porovnaní so sekvenciou ITS v databáze. ITS oblasti tak potvrdzujú svoju využiteľnosť v efektívnych analýzach genómu jakonu.
- The internal transcribed spacer (ITS) region of ribosomal DNA (rDNA) has proven to be a useful tool for phylogenetic studies in many angiosperm families. For this reason we have decided to use ITS regions as molecular markers for the evaluation of phylogenetic relationships in yacon (Smallanthus sonchifolius, Poepp. and Endl.). The aim of our work was the optimization of a PCR protocol for the amplification of the region between the DNA coding the large and the small RNA subunit, and the comparison of sequences obtained. Amplicons obtained from three Bolivian yacon landraces were sequenced. Sequences obtained were alligned with ITS region present in the database. The analysed ITS regions of Bolivian yacon genotypes showed differences in the nucleotides 163-164 and 235-236. ITS region has confirmed their potential in the effective yacon genome analyses. (en)
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Title
| - ITS regions in molecular studies of genetic resources of yacon (en)
- ITS úseky v molekulárnom hodnotení genetických zdrojov jakonu
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skos:prefLabel
| - ITS regions in molecular studies of genetic resources of yacon (en)
- ITS úseky v molekulárnom hodnotení genetických zdrojov jakonu
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skos:notation
| - RIV/60460709:41610/12:55119!RIV13-MSM-41610___
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http://linked.open...avai/predkladatel
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/60460709:41610/12:55119
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - yacon, ITS region, molecular diversity, sequence (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Bežo, prof. RNDr. CSc., Milan
- Fernández Cusimamani, Eloy
- Russso, Ing, Daniela
- Štefúnová, Ing. Ph.D., Veronika
- Žiarovská, Ing. PaedDr. Ph.D., Jana
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http://linked.open...ain/vavai/riv/wos
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issn
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number of pages
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http://localhost/t...ganizacniJednotka
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is http://linked.open...avai/riv/vysledek
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