About: Biochanin A and daidzein influence meiotic maturation of pig oocytes in different manner     Goto   Sponge   NotDistinct   Permalink

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  • The aim of the study was to determine the influence of different concentrations of phytoestrogens biochanin A (BIO A; 20, 40, 50µg ml–1) and daidzein (DAI; 10, 20, 40, 50µg ml–1) on the course of meiotic maturation of pig oocytes. After a 24-hour cultivation, a stage of nuclear maturation was achieved and the areas of cumulus-oocyte complexes (COCs), as an indicator of cumulus expansion, were evaluated. The effects of both contaminants on oocytes were mani - fested from the lowest concentration used. Nuclear maturation was inhibited in a dose-dependent manner in the case of BIO A. Effects of DAI reached a plateau at a concentration of 20µg ml–1.Possible relationship to limited solubility of DAI was excluded because limits of DAI solubility in culture medium were confirmed at 50 µg ml–1.The cumulus expansion was also influenced in a different manner – reduction of the COC’s area by BIO A was dose-dependent, whereas DAI had the strongest effect on CCs in the lowest and highest concentrations us
  • The aim of the study was to determine the influence of different concentrations of phytoestrogens biochanin A (BIO A; 20, 40, 50µg ml–1) and daidzein (DAI; 10, 20, 40, 50µg ml–1) on the course of meiotic maturation of pig oocytes. After a 24-hour cultivation, a stage of nuclear maturation was achieved and the areas of cumulus-oocyte complexes (COCs), as an indicator of cumulus expansion, were evaluated. The effects of both contaminants on oocytes were mani - fested from the lowest concentration used. Nuclear maturation was inhibited in a dose-dependent manner in the case of BIO A. Effects of DAI reached a plateau at a concentration of 20µg ml–1.Possible relationship to limited solubility of DAI was excluded because limits of DAI solubility in culture medium were confirmed at 50 µg ml–1.The cumulus expansion was also influenced in a different manner – reduction of the COC’s area by BIO A was dose-dependent, whereas DAI had the strongest effect on CCs in the lowest and highest concentrations us (en)
  • The aim of the study was to determine the influence of different concentrations of phytoestrogens biochanin A (BIO A; 20, 40, 50µg ml–1) and daidzein (DAI; 10, 20, 40, 50µg ml–1) on the course of meiotic maturation of pig oocytes. After a 24-hour cultivation, a stage of nuclear maturation was achieved and the areas of cumulus-oocyte complexes (COCs), as an indicator of cumulus expansion, were evaluated. The effects of both contaminants on oocytes were mani - fested from the lowest concentration used. Nuclear maturation was inhibited in a dose-dependent manner in the case of BIO A. Effects of DAI reached a plateau at a concentration of 20µg ml–1.Possible relationship to limited solubility of DAI was excluded because limits of DAI solubility in culture medium were confirmed at 50 µg ml–1.The cumulus expansion was also influenced in a different manner – reduction of the COC’s area by BIO A was dose-dependent, whereas DAI had the strongest effect on CCs in the lowest and highest concentrations us (cs)
Title
  • Biochanin A and daidzein influence meiotic maturation of pig oocytes in different manner
  • Biochanin A and daidzein influence meiotic maturation of pig oocytes in different manner (en)
  • Biochanin A and daidzein influence meiotic maturation of pig oocytes in different manner (cs)
skos:prefLabel
  • Biochanin A and daidzein influence meiotic maturation of pig oocytes in different manner
  • Biochanin A and daidzein influence meiotic maturation of pig oocytes in different manner (en)
  • Biochanin A and daidzein influence meiotic maturation of pig oocytes in different manner (cs)
skos:notation
  • RIV/60460709:41210/14:64334!RIV15-MSM-41210___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • S
http://linked.open...iv/cisloPeriodika
  • 3
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 5292
http://linked.open...ai/riv/idVysledku
  • RIV/60460709:41210/14:64334
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • reproduction, sow, soya, phytoestrogens, meiosis (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • CZ - Česká republika
http://linked.open...ontrolniKodProRIV
  • [4B4F2CE0067D]
http://linked.open...i/riv/nazevZdroje
  • Scientia Agriculturae Bohemica
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 45
http://linked.open...iv/tvurceVysledku
  • Jílek, František
  • Rajmon, Radko
  • Křivohlávková, Lenka
  • Drábek, Ondřej
  • Hošková, Kristýna
  • Kadlečková, Linda
http://linked.open...ain/vavai/riv/wos
  • 0
issn
  • 1211-3174
number of pages
http://localhost/t...ganizacniJednotka
  • 41210
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