About: Zinc induces DNA damage in tobacco roots

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	We applied the alkaline version of the single-cell gel electrophoresis (comet assay) to seedlings of heterozygous tobacco (Nicotiana tabacum L. var. xanthi) treated with zinc acetate dihydrate (20 to 80 mM Zn2+ for 2 h or 2 to 12 mM Zn2+ for 24 h). A dose dependent increase in DNA damage expressed by the tail moment values were observed in nuclei isolated from the roots after 2 and 24 h Zn2+ treatments. In contrast, Zn2+ did not induce significant DNA damage to leaf nuclei, with the exception of 10 or 12 mM Zn2+ for 24 h. Somatic mutations, identified as dark green, yellow, and dark green/yellow double sectors on the pale green tobacco leaves were not detected after any Zn2+ treatments. The accumulation of Zn in roots and shoots was determined by inductively coupled plasma optical emission spectrometry and Zn content in roots was about three times higher than in shoots. We applied the alkaline version of the single-cell gel electrophoresis (comet assay) to seedlings of heterozygous tobacco (Nicotiana tabacum L. var. xanthi) treated with zinc acetate dihydrate (20 to 80 mM Zn2+ for 2 h or 2 to 12 mM Zn2+ for 24 h). A dose dependent increase in DNA damage expressed by the tail moment values were observed in nuclei isolated from the roots after 2 and 24 h Zn2+ treatments. In contrast, Zn2+ did not induce significant DNA damage to leaf nuclei, with the exception of 10 or 12 mM Zn2+ for 24 h. Somatic mutations, identified as dark green, yellow, and dark green/yellow double sectors on the pale green tobacco leaves were not detected after any Zn2+ treatments. The accumulation of Zn in roots and shoots was determined by inductively coupled plasma optical emission spectrometry and Zn content in roots was about three times higher than in shoots. (en)
Title	Zinc induces DNA damage in tobacco roots Zinc induces DNA damage in tobacco roots (en)
skos:prefLabel	Zinc induces DNA damage in tobacco roots Zinc induces DNA damage in tobacco roots (en)
skos:notation	RIV/60460709:41210/13:61981!RIV14-GA0-41210___
http://linked.open...avai/predkladatel	Fakulta agrobiologie, potravinových a přírodních zdrojů
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GAP501/11/1239), Z(AV0Z50380511)
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Száková, Jiřina Pavlíková, Daniela
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Česká zemědělská univerzita v Praze / Fakulta agrobiologie, potravinových a přírodních zdrojů
http://linked.open...dnocenehoVysledku	118773
http://linked.open...ai/riv/idVysledku	RIV/60460709:41210/13:61981
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	comet assay, ethyl methanesulphonate, Nicotiana tabacum, somatic mutations (en)
http://linked.open.../riv/klicoveSlovo	ethyl methanesulphonate comet assay somatic mutations Nicotiana tabacum
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[9B43C130B2BB]
http://linked.open...i/riv/nazevZdroje	Biologia Plantarum
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Nitrosative stress during natural and zinc induced senescence
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	57
http://linked.open...iv/tvurceVysledku	Procházková, Dagmar Száková, Jiřina Pavlíková, Daniela Wilhelmová, Naďa Gichner, Tomáš
http://linked.open...ain/vavai/riv/wos	000324878300025
http://linked.open...n/vavai/riv/zamer	Mechanismy regulace růstu a vývoje rostlin na úrovni buněk, orgánů a celých organismů: fyziologické, genetické a molekulárně biologické základy
issn	0006-3134
number of pages	5 (xsd:int)
http://localhost/t...ganizacniJednotka	41210

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