| Attributes | Values |
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| rdf:type
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| Description
| - Apricot latent virus (ALV) is one of viruses infecting apricots mainly in Mediterranean countries. It is included in certification schemes of EPPO. That is why we adapted a PCR method for its detection. Positive control was obtained from Dr. Myrta from Italy. Buds from these sprouts were budded onto GF 305 rootstock in a greenhouse. Under these conditions these plants display no symptoms but they contain the virus. RNA from leaves was extracted by silica method. Primers were designed on the basis of sequences from NCBI database. Predicted product lenght is 206 bp. Transcription is done using AMV RT at 42 °C. Conditions for PCR were optimized (72 °C for 5 min, 35 cycles of 94 °C, 50 °C and 72 °C for denaturation, annealing and DNA synthesis, respectively, and final 72 °C for 4 min). Molecular hybridization using DNA and RNA probes labeled by digoxygeninhas also been optimised. These methods were used for testing of leaf samples from apricot trees from orchards in the Czech Republic. Until now no infec
- Apricot latent virus (ALV) is one of viruses infecting apricots mainly in Mediterranean countries. It is included in certification schemes of EPPO. That is why we adapted a PCR method for its detection. Positive control was obtained from Dr. Myrta from Italy. Buds from these sprouts were budded onto GF 305 rootstock in a greenhouse. Under these conditions these plants display no symptoms but they contain the virus. RNA from leaves was extracted by silica method. Primers were designed on the basis of sequences from NCBI database. Predicted product lenght is 206 bp. Transcription is done using AMV RT at 42 °C. Conditions for PCR were optimized (72 °C for 5 min, 35 cycles of 94 °C, 50 °C and 72 °C for denaturation, annealing and DNA synthesis, respectively, and final 72 °C for 4 min). Molecular hybridization using DNA and RNA probes labeled by digoxygeninhas also been optimised. These methods were used for testing of leaf samples from apricot trees from orchards in the Czech Republic. Until now no infec (en)
- Apricot latent virus (ALV) is one of viruses infecting apricots mainly in Mediterranean countries. It is included in certification schemes of EPPO. That is why we adapted a PCR method for its detection. Positive control was obtained from Dr. Myrta from Italy. Buds from these sprouts were budded onto GF 305 rootstock in a greenhouse. Under these conditions these plants display no symptoms but they contain the virus. RNA from leaves was extracted by silica method. Primers were designed on the basis of sequences from NCBI database. Predicted product lenght is 206 bp. Transcription is done using AMV RT at 42 °C. Conditions for PCR were optimized (72 °C for 5 min, 35 cycles of 94 °C, 50 °C and 72 °C for denaturation, annealing and DNA synthesis, respectively, and final 72 °C for 4 min). Molecular hybridization using DNA and RNA probes labeled by digoxygeninhas also been optimised. These methods were used for testing of leaf samples from apricot trees from orchards in the Czech Republic. Until now no infec (cs)
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| Title
| - STUDIES OF APRICOT LATENT VIRUS IN THE CZECH REPUBLIC
- Studium latentního viru meruňky v ČR (cs)
- STUDIES OF APRICOT LATENT VIRUS IN THE CZECH REPUBLIC (en)
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| skos:prefLabel
| - STUDIES OF APRICOT LATENT VIRUS IN THE CZECH REPUBLIC
- Studium latentního viru meruňky v ČR (cs)
- STUDIES OF APRICOT LATENT VIRUS IN THE CZECH REPUBLIC (en)
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| skos:notation
| - RIV/60460709:41210/08:26701!RIV09-MSM-41210___
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/60460709:41210/08:26701
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - Apricot latent virus, PCR, molecular hybridization, detection, apricot (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Zouhar, Miloslav
- Ryšánek, Pavel
- Grimová, Lenka
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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| http://localhost/t...ganizacniJednotka
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| is http://linked.open...avai/riv/vysledek
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