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  • Cultivation methods for the quantitative detection of the faecal bacteria are inaccurate and time consuming, also not all bacteria are cultivatable and results obtained may be incorrect. Hence, the aim of our study was to evaluate FISH method for the quantitative detection of different bacterial group in faecal samples of infants, calves, and lambs during the milk-feeding period. Faecal samples from 91 infants, 20 calves, and 18 lambs were tested. A specific fluorescence in situ hybridisation (FISH) kit for Bifidobacterium spp., Clostridium butyricum group, Escherichia coli, and Lactobacillus spp. were used for the quantitative detection of bacteria compared to cultivation methods. Sixty-nine infants possessed high numbers of bifidobacteria in their faeces. Cultivation counts were nonsignificantly lower (9.91 ± 0.67) than counts determinated by FISH (9.92 ± 0.55). Twenty-two infants did not contain detectable bifidobacteria. These infants possessed as a major group of their faecal f
  • Cultivation methods for the quantitative detection of the faecal bacteria are inaccurate and time consuming, also not all bacteria are cultivatable and results obtained may be incorrect. Hence, the aim of our study was to evaluate FISH method for the quantitative detection of different bacterial group in faecal samples of infants, calves, and lambs during the milk-feeding period. Faecal samples from 91 infants, 20 calves, and 18 lambs were tested. A specific fluorescence in situ hybridisation (FISH) kit for Bifidobacterium spp., Clostridium butyricum group, Escherichia coli, and Lactobacillus spp. were used for the quantitative detection of bacteria compared to cultivation methods. Sixty-nine infants possessed high numbers of bifidobacteria in their faeces. Cultivation counts were nonsignificantly lower (9.91 ± 0.67) than counts determinated by FISH (9.92 ± 0.55). Twenty-two infants did not contain detectable bifidobacteria. These infants possessed as a major group of their faecal f (en)
  • Cultivation methods for the quantitative detection of the faecal bacteria are inaccurate and time consuming, also not all bacteria are cultivatable and results obtained may be incorrect. Hence, the aim of our study was to evaluate FISH method for the quantitative detection of different bacterial group in faecal samples of infants, calves, and lambs during the milk-feeding period. Faecal samples from 91 infants, 20 calves, and 18 lambs were tested. A specific fluorescence in situ hybridisation (FISH) kit for Bifidobacterium spp., Clostridium butyricum group, Escherichia coli, and Lactobacillus spp. were used for the quantitative detection of bacteria compared to cultivation methods. Sixty-nine infants possessed high numbers of bifidobacteria in their faeces. Cultivation counts were nonsignificantly lower (9.91 ± 0.67) than counts determinated by FISH (9.92 ± 0.55). Twenty-two infants did not contain detectable bifidobacteria. These infants possessed as a major group of their faecal f (cs)
Title
  • Detection of faecal bacteria by FISH method.
  • Detection of faecal bacteria by FISH method. (en)
  • Detekce fekálních bakterií FISH metodou. (cs)
skos:prefLabel
  • Detection of faecal bacteria by FISH method.
  • Detection of faecal bacteria by FISH method. (en)
  • Detekce fekálních bakterií FISH metodou. (cs)
skos:notation
  • RIV/60460709:41210/06:17258!RIV07-GA0-41210___
http://linked.open.../vavai/riv/strany
  • 0;0
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GP523/05/P117), Z(MSM6046070901)
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 471016
http://linked.open...ai/riv/idVysledku
  • RIV/60460709:41210/06:17258
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • faecal bacteria, FISH method, bifidobacteria (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [52B01277E3BA]
http://linked.open...v/mistoKonaniAkce
  • Prague
http://linked.open...i/riv/mistoVydani
  • Prague
http://linked.open...vEdiceCisloSvazku
  • Neuvedeno, 0
http://linked.open...i/riv/nazevZdroje
  • Book of Abstracts Genetics of industrial microorganisms.
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...iv/tvurceVysledku
  • Killer, Jiří
  • Tománková, Eva
  • Rada, Vojtěch
  • Trojanová, Iva
http://linked.open...vavai/riv/typAkce
http://linked.open.../riv/zahajeniAkce
http://linked.open...n/vavai/riv/zamer
number of pages
http://purl.org/ne...btex#hasPublisher
  • Mikrobiologický ústav AV ČR
https://schema.org/isbn
  • 80-239-7323-1
http://localhost/t...ganizacniJednotka
  • 41210
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