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  • Pig oocytes matured in vitro are parthenogenetically actived (70%) after the treatment with 2mM NO-donor SNAP for 10 hours. The chelation of intracellular calcium ions after the treatment with BAPTA-AM suppressed the SNAP-induced activation in dose-dependent manner (56% activated oocytes after the treatment with 5 mikroM BAPTA-AM; non activated oocyte after the treatment with 50 mikroM BAPTA-AM). Activation by NOdonor is dependent on the influx of calcium from extracellular spaces, because the blockage of calcium channels by verapamil significantly reduced the activation rate SNAP-treated oocytes (17% of activated oocytes after the treatment with 0,08 mM verapamil). These data indicate that the activation of pig oocytes with NO-donor is calcium-dependent. The calcium for the activation is mobilized from extracellular and intracellular stores.
  • Pig oocytes matured in vitro are parthenogenetically actived (70%) after the treatment with 2mM NO-donor SNAP for 10 hours. The chelation of intracellular calcium ions after the treatment with BAPTA-AM suppressed the SNAP-induced activation in dose-dependent manner (56% activated oocytes after the treatment with 5 mikroM BAPTA-AM; non activated oocyte after the treatment with 50 mikroM BAPTA-AM). Activation by NOdonor is dependent on the influx of calcium from extracellular spaces, because the blockage of calcium channels by verapamil significantly reduced the activation rate SNAP-treated oocytes (17% of activated oocytes after the treatment with 0,08 mM verapamil). These data indicate that the activation of pig oocytes with NO-donor is calcium-dependent. The calcium for the activation is mobilized from extracellular and intracellular stores. (en)
  • Pig oocytes matured in vitro are parthenogenetically actived (70%) after the treatment with 2mM NO-donor SNAP for 10 hours. The chelation of intracellular calcium ions after the treatment with BAPTA-AM suppressed the SNAP-induced activation in dose-dependent manner (56% activated oocytes after the treatment with 5 mikroM BAPTA-AM; non activated oocyte after the treatment with 50 mikroM BAPTA-AM). Activation by NOdonor is dependent on the influx of calcium from extracellular spaces, because the blockage of calcium channels by verapamil significantly reduced the activation rate SNAP-treated oocytes (17% of activated oocytes after the treatment with 0,08 mM verapamil). These data indicate that the activation of pig oocytes with NO-donor is calcium-dependent. The calcium for the activation is mobilized from extracellular and intracellular stores. (cs)
Title
  • The role of extracellular and intracellular calcium stores in NO-dependent parthenogenetic activation of pig oocytes
  • Úloha extracelárních a intracelulárních vápníkových depozit při NO-dependentní partenogenetické aktivaci prasečích oocytů (cs)
  • The role of extracellular and intracellular calcium stores in NO-dependent parthenogenetic activation of pig oocytes (en)
skos:prefLabel
  • The role of extracellular and intracellular calcium stores in NO-dependent parthenogenetic activation of pig oocytes
  • Úloha extracelárních a intracelulárních vápníkových depozit při NO-dependentní partenogenetické aktivaci prasečích oocytů (cs)
  • The role of extracellular and intracellular calcium stores in NO-dependent parthenogenetic activation of pig oocytes (en)
skos:notation
  • RIV/60460709:41210/05:11691!RIV06-MSM-41210___
http://linked.open.../vavai/riv/strany
  • 265;268
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(MSM6046070901)
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 541402
http://linked.open...ai/riv/idVysledku
  • RIV/60460709:41210/05:11691
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • pig, oocyte, meiotic maturation, activation, calcium (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [3ABB9903077F]
http://linked.open...v/mistoKonaniAkce
  • České Budějovice
http://linked.open...i/riv/mistoVydani
  • České Budějovice
http://linked.open...vEdiceCisloSvazku
  • Neuvedeno, 0
http://linked.open...i/riv/nazevZdroje
  • Current Problems of Breeding, Health, Growth and Production Pigs, České Budějovice
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...iv/tvurceVysledku
  • Petr, Jaroslav
  • Ješeta, Michal
  • Chmelíková, Eva
  • Rozinek, Jiří
  • Kuthanová, Zuzana
http://linked.open...vavai/riv/typAkce
http://linked.open.../riv/zahajeniAkce
http://linked.open...n/vavai/riv/zamer
number of pages
http://purl.org/ne...btex#hasPublisher
  • Biologická fakulta
https://schema.org/isbn
  • 80-85645-50-5
http://localhost/t...ganizacniJednotka
  • 41210
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