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rdf:type
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Description
| - Nitric oxide synthase (NOS) has been considered as a highly regulated enzyme that can produce nitric oxide (NO) from molecular oxygen and L-arginine. Three isoforms of NOS are known neuronal, inducible and endothelial. NO is an important signalling molecule that mediates many physiological processes. The aim of our study was to assess whether NOS is involved in porcine oocytes meiotic maturation by NO production. The expression of NOS was studied in three stages of porcine oocytes meiotic maturation, germinal vesicle (0 h), first meiotic metaphase (24 h) and second meiotic metaphase stage (48 h), using western blotting method. The activity of NOS was observed by measurement of NO metabolites, nitrites and nitrates, with Griess reagents. Two NOS isoforms were detected in porcine oocytes: inducible and endothelial nitric oxide synthase. These isoforms were detected in all three stages of porcine oocyte meiotic maturation. The results of NO measurement showed that the amount of NO metabolites signi
- Nitric oxide synthase (NOS) has been considered as a highly regulated enzyme that can produce nitric oxide (NO) from molecular oxygen and L-arginine. Three isoforms of NOS are known neuronal, inducible and endothelial. NO is an important signalling molecule that mediates many physiological processes. The aim of our study was to assess whether NOS is involved in porcine oocytes meiotic maturation by NO production. The expression of NOS was studied in three stages of porcine oocytes meiotic maturation, germinal vesicle (0 h), first meiotic metaphase (24 h) and second meiotic metaphase stage (48 h), using western blotting method. The activity of NOS was observed by measurement of NO metabolites, nitrites and nitrates, with Griess reagents. Two NOS isoforms were detected in porcine oocytes: inducible and endothelial nitric oxide synthase. These isoforms were detected in all three stages of porcine oocyte meiotic maturation. The results of NO measurement showed that the amount of NO metabolites signi (en)
- Nitric oxide synthase (NOS) has been considered as a highly regulated enzyme that can produce nitric oxide (NO) from molecular oxygen and L-arginine. Three isoforms of NOS are known neuronal, inducible and endothelial. NO is an important signalling molecule that mediates many physiological processes. The aim of our study was to assess whether NOS is involved in porcine oocytes meiotic maturation by NO production. The expression of NOS was studied in three stages of porcine oocytes meiotic maturation, germinal vesicle (0 h), first meiotic metaphase (24 h) and second meiotic metaphase stage (48 h), using western blotting method. The activity of NOS was observed by measurement of NO metabolites, nitrites and nitrates, with Griess reagents. Two NOS isoforms were detected in porcine oocytes: inducible and endothelial nitric oxide synthase. These isoforms were detected in all three stages of porcine oocyte meiotic maturation. The results of NO measurement showed that the amount of NO metabolites signi (cs)
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Title
| - Nitric Oxide Synthase and Nitric Oxide Importance for Meiotic Maturation of Pig Oocytes
- Význam NO-syntázy a oxidu dusnatého pro meiotické zrání oocytu prasete (cs)
- Nitric Oxide Synthase and Nitric Oxide Importance for Meiotic Maturation of Pig Oocytes (en)
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skos:prefLabel
| - Nitric Oxide Synthase and Nitric Oxide Importance for Meiotic Maturation of Pig Oocytes
- Význam NO-syntázy a oxidu dusnatého pro meiotické zrání oocytu prasete (cs)
- Nitric Oxide Synthase and Nitric Oxide Importance for Meiotic Maturation of Pig Oocytes (en)
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skos:notation
| - RIV/60460709:41210/05:11536!RIV06-GA0-41210___
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http://linked.open.../vavai/riv/strany
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - P(GD523/03/H076), Z(MSM6046070901)
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/60460709:41210/05:11536
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - pig, oocyte, meiotic maturation, NOS, NO (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...ontrolniKodProRIV
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http://linked.open...v/mistoKonaniAkce
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http://linked.open...i/riv/mistoVydani
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http://linked.open...vEdiceCisloSvazku
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http://linked.open...i/riv/nazevZdroje
| - Journal of Applied Biomedicine
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...iv/tvurceVysledku
| - Jílek, František
- Petr, Jaroslav
- Ješeta, Michal
- Chmelíková, Eva
- Sedmíková, Markéta
- Lánská, Vilma
- Rozinek, Jiří
- Kuthanová, Zuzana
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http://linked.open...vavai/riv/typAkce
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http://linked.open.../riv/zahajeniAkce
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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http://purl.org/ne...btex#hasPublisher
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http://localhost/t...ganizacniJednotka
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is http://linked.open...avai/riv/vysledek
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