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Description
  • Tobacco, Nicotiana tabacum L., var. Wisconsin 38 as the control (WSC), and a genetically modified line of the same variety, bearing the transgene coding for the polyhistidine cluster, combined with yeast metallothionein (HisCUP) was tested for cadmium accumulation and tolerance. The screening test was conducted on sand nutrient medium with addition of Knop’s nutrient solution modified by addition of various cadmium doses. Significantly increasing Cd accumulation in above ground biomass of HisCUP tobacco growing in Cd concentration 0.2 and 0.6 mg Cd.l-1 of nutrient solution was determined comparing to the control. A dose of 5.4 mg Cd.l-1 induced cadmium toxicity symptoms of control tobacco. The highest dose of Cd was lethal for this treatment. HisCUP showed increasing plant resistance against stress response induced by cadmium. The experiment was continued in model pot experiments with two various polluted soils. The Cd content in the above ground biomass of transgenic tobacco was increased by 45-75 %
  • Tobacco, Nicotiana tabacum L., var. Wisconsin 38 as the control (WSC), and a genetically modified line of the same variety, bearing the transgene coding for the polyhistidine cluster, combined with yeast metallothionein (HisCUP) was tested for cadmium accumulation and tolerance. The screening test was conducted on sand nutrient medium with addition of Knop’s nutrient solution modified by addition of various cadmium doses. Significantly increasing Cd accumulation in above ground biomass of HisCUP tobacco growing in Cd concentration 0.2 and 0.6 mg Cd.l-1 of nutrient solution was determined comparing to the control. A dose of 5.4 mg Cd.l-1 induced cadmium toxicity symptoms of control tobacco. The highest dose of Cd was lethal for this treatment. HisCUP showed increasing plant resistance against stress response induced by cadmium. The experiment was continued in model pot experiments with two various polluted soils. The Cd content in the above ground biomass of transgenic tobacco was increased by 45-75 % (en)
  • Tobacco, Nicotiana tabacum L., var. Wisconsin 38 as the control (WSC), and a genetically modified line of the same variety, bearing the transgene coding for the polyhistidine cluster, combined with yeast metallothionein (HisCUP) was tested for cadmium accumulation and tolerance. The screening test was conducted on sand nutrient medium with addition of Knop’s nutrient solution modified by addition of various cadmium doses. Significantly increasing Cd accumulation in above ground biomass of HisCUP tobacco growing in Cd concentration 0.2 and 0.6 mg Cd.l-1 of nutrient solution was determined comparing to the control. A dose of 5.4 mg Cd.l-1 induced cadmium toxicity symptoms of control tobacco. The highest dose of Cd was lethal for this treatment. HisCUP showed increasing plant resistance against stress response induced by cadmium. The experiment was continued in model pot experiments with two various polluted soils. The Cd content in the above ground biomass of transgenic tobacco was increased by 45-75 % (cs)
Title
  • Cadmium tolerance and accumulation in transgenic tobacco plants with a yeast metallothionein combined with a polyhistidine tail (cs)
  • Schopnost transgenního tabáku připraveného kombinací genu pro metallothionein a genu pro histidinovou kotvu akumulovat a tolerovat kadmium
  • Schopnost transgenního tabáku připraveného kombinací genu pro metallothionein a genu pro histidinovou kotvu akumulovat a tolerovat kadmium (en)
skos:prefLabel
  • Cadmium tolerance and accumulation in transgenic tobacco plants with a yeast metallothionein combined with a polyhistidine tail (cs)
  • Schopnost transgenního tabáku připraveného kombinací genu pro metallothionein a genu pro histidinovou kotvu akumulovat a tolerovat kadmium
  • Schopnost transgenního tabáku připraveného kombinací genu pro metallothionein a genu pro histidinovou kotvu akumulovat a tolerovat kadmium (en)
skos:notation
  • RIV/60460709:41210/04:7847!RIV/2005/GA0/412105/N
http://linked.open.../vavai/riv/strany
  • 233;237
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA526/02/0293)
http://linked.open...iv/cisloPeriodika
  • 0
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 585550
http://linked.open...ai/riv/idVysledku
  • RIV/60460709:41210/04:7847
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • transgenic tobacco, metallothionein, polyhistidine, cadmium, accumulation, tolerance (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [FFC2FEC511B2]
http://linked.open...i/riv/nazevZdroje
  • International Biodeterioration & Biodegradation
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 54
http://linked.open...iv/tvurceVysledku
  • Macek, Tomáš
  • Macková, Martina
  • Száková, Jiřina
  • Balík, Jiří
  • Pavlíková, Daniela
issn
  • 0964-8305
number of pages
http://localhost/t...ganizacniJednotka
  • 41210
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