About: Molecular Markers of Apple Trees Resistance against Scab (Venturia inaequalis CKE.)

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About: Molecular Markers of Apple Trees Resistance against Scab (Venturia inaequalis CKE.) Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	A set of dominant and co-dominant PCR markers was used to detect the Vf alleles. This methodological procedure was verified on a collection of apple tree varieties with a declared resistance against scab, determined by the dominant Vf gene. Nine reference varieties containing the Vf gene were used, developed by the Research and Breeding Institute of Pomology in Holovousy. Conducted was also a segregation analysis of seedlings of eight complex combination crossbreeds. The resistance or sensitivity of theassessed plant material was also classified in standard greenhouse infection tests. The so-called multi-PCR was used to detect alleles of the Vf gene. The actual amplification and sequences of primer pairs were obtained from Tartarini et al. (1999) exp eriments. The multi-PCR used allowed a simultaneous amplification of one dominant and one co-dominant marker. The co-dominant marker enabled the dominant homozygotes, heterozygotes and recessive homozygotes to be distinguished from each other. The domina A set of dominant and co-dominant PCR markers was used to detect the Vf alleles. This methodological procedure was verified on a collection of apple tree varieties with a declared resistance against scab, determined by the dominant Vf gene. Nine reference varieties containing the Vf gene were used, developed by the Research and Breeding Institute of Pomology in Holovousy. Conducted was also a segregation analysis of seedlings of eight complex combination crossbreeds. The resistance or sensitivity of theassessed plant material was also classified in standard greenhouse infection tests. The so-called multi-PCR was used to detect alleles of the Vf gene. The actual amplification and sequences of primer pairs were obtained from Tartarini et al. (1999) exp eriments. The multi-PCR used allowed a simultaneous amplification of one dominant and one co-dominant marker. The co-dominant marker enabled the dominant homozygotes, heterozygotes and recessive homozygotes to be distinguished from each other. The domina (en)
Title	Molecular Markers of Apple Trees Resistance against Scab (Venturia inaequalis CKE.) Molecular Markers of Apple Trees Resistance against Scab (Venturia inaequalis CKE.) (en)
skos:prefLabel	Molecular Markers of Apple Trees Resistance against Scab (Venturia inaequalis CKE.) Molecular Markers of Apple Trees Resistance against Scab (Venturia inaequalis CKE.) (en)
skos:notation	RIV/60460709:41210/01:00002909!RIV/2002/MSM/412102/N
http://linked.open.../vavai/riv/strany	49
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM 412100002)
http://linked.open...vai/riv/dodaniDat	2002
http://linked.open...aciTvurceVysledku	Skupinová, Sylva Vejl, Pavel Blažek, Pavel
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Česká zemědělská univerzita v Praze / Fakulta agrobiologie, potravinových a přírodních zdrojů
http://linked.open...dnocenehoVysledku	687576
http://linked.open...ai/riv/idVysledku	RIV/60460709:41210/01:00002909
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Apple, (Malus sp.), scab, (Venturia inaequalis CKE.), DNA, molecular markers, multi PCR, Vf gene (en)
http://linked.open.../riv/klicoveSlovo	molecular markers scab DNA Apple (Malus sp.) (Venturia inaequalis CKE.) Vf gene multi PCR
http://linked.open...ontrolniKodProRIV	[54298B9F72E0]
http://linked.open...v/mistoKonaniAkce	Třeboň
http://linked.open...i/riv/mistoVydani	České Budějovice
http://linked.open...i/riv/nazevZdroje	4th International Symposium in the Series Recent Advances in Plant Biotechnology, Plant Molecular Biology for the New Millennium, Book of Abstracts
http://linked.open...in/vavai/riv/obor	GE
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...UplatneniVysledku	2001
http://linked.open...iv/tvurceVysledku	Vejl, Pavel Blažek, Pavel Drahošová, H. Skupinová, Sylva
http://linked.open...vavai/riv/typAkce	EUR - Evropská
http://linked.open.../riv/zahajeniAkce	2001-01-01 (xsd:date)
http://linked.open...n/vavai/riv/zamer	http://linked.opendata.cz/resource/domain/vavai/zamer/MSM%20412100002
number of pages	1 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Ústav molekulární biologie rostlin AV ČR
http://localhost/t...ganizacniJednotka	41210

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