About: Detection of Mi Gene in Tomato (Lycopersicon esculentum)

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	This paper presents the scope of using co-dominant DNA markers in the detection of Mi gene of the Lycopersicon esculentum, resistant against parasitic soil nematodes of the Meloidogyne incognita. In this experiment was assessed for gene sources - varieties of the F1 generation type and the segregating F2 generation. DNA was isolated from the meristematic tissues. For the dominantly established tomato's resistance against Meloidogyne incognita was used a co-dominant CAPS marker of the Mi gene. Dominant and recessive alleles were detected after the digestion of the primary PCR product by the restriction enzyme TaqI. The identified PCR resistance markers in the Lycopersicon esculentum were confronted with the results of standard infection tests. Heterozygous sets of the Mi gene were detected in two species of the F1 hybrid type. The co-dominant CAPS marker enabled an unambiguous distinction between heterozygotes and dominant and recessive homozygotes. In tomato self-pollination occurred, and an F2 genera This paper presents the scope of using co-dominant DNA markers in the detection of Mi gene of the Lycopersicon esculentum, resistant against parasitic soil nematodes of the Meloidogyne incognita. In this experiment was assessed for gene sources - varieties of the F1 generation type and the segregating F2 generation. DNA was isolated from the meristematic tissues. For the dominantly established tomato's resistance against Meloidogyne incognita was used a co-dominant CAPS marker of the Mi gene. Dominant and recessive alleles were detected after the digestion of the primary PCR product by the restriction enzyme TaqI. The identified PCR resistance markers in the Lycopersicon esculentum were confronted with the results of standard infection tests. Heterozygous sets of the Mi gene were detected in two species of the F1 hybrid type. The co-dominant CAPS marker enabled an unambiguous distinction between heterozygotes and dominant and recessive homozygotes. In tomato self-pollination occurred, and an F2 genera (en)
Title	Detection of Mi Gene in Tomato (Lycopersicon esculentum) Detection of Mi Gene in Tomato (Lycopersicon esculentum) (en)
skos:prefLabel	Detection of Mi Gene in Tomato (Lycopersicon esculentum) Detection of Mi Gene in Tomato (Lycopersicon esculentum) (en)
skos:notation	RIV/60460709:41210/01:00002744!RIV/2002/MSM/412102/N
http://linked.open.../vavai/riv/strany	81
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM 412100002)
http://linked.open...vai/riv/dodaniDat	2002
http://linked.open...aciTvurceVysledku	Skupinová, Sylva Vejl, Pavel Zouhar, Miloslav
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Česká zemědělská univerzita v Praze / Fakulta agrobiologie, potravinových a přírodních zdrojů
http://linked.open...dnocenehoVysledku	677314
http://linked.open...ai/riv/idVysledku	RIV/60460709:41210/01:00002744
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Tomato, (Lycopersicon esculentum), Mi gene, PCR markers, CAPS markers (en)
http://linked.open.../riv/klicoveSlovo	Tomato CAPS markers (Lycopersicon esculentum) Mi gene PCR markers
http://linked.open...ontrolniKodProRIV	[9582BDD1EB63]
http://linked.open...v/mistoKonaniAkce	Praha
http://linked.open...i/riv/mistoVydani	Praha
http://linked.open...i/riv/nazevZdroje	Crop Science on the Verge of the 21st Century - Opportunities and Challenges.
http://linked.open...in/vavai/riv/obor	GE
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...UplatneniVysledku	2001
http://linked.open...iv/tvurceVysledku	Vejl, Pavel Zouhar, Miloslav Skupinová, Sylva Klápště, P. Srbek, L.
http://linked.open...vavai/riv/typAkce	EUR - Evropská
http://linked.open.../riv/zahajeniAkce	2001-01-01 (xsd:date)
http://linked.open...n/vavai/riv/zamer	http://linked.opendata.cz/resource/domain/vavai/zamer/MSM%20412100002
number of pages	1 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Institute of crop production
https://schema.org/isbn	80-86555-01-1
http://localhost/t...ganizacniJednotka	41210

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