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  • As model botanic species were selected tomato (Lycopersicon esculentum) and potato (Solanum tuberosum ssp. tuberosum). The potato was assessed for the collection of tetraploidic gene sources. The tomato was assessed for gene sources - varieties of the F1generation type and the segregating F2 generation. The potato's resistance against Ro1 of the Globodera rostochiensis pathotype was marked with a PCR amplification of the Gro 1.2.3 loci in chromosome II.. To assess the resistance, the results of experim ents conducted by Leister et al. (1997) were used. One loci of quantitative resistant - PCR marker has a close gene link with the monitored resistance. For the dominantly established tomato's resistance against Meloidogyne incognita was used a co-dominant CAPS marker of the Mi gene. The experiments were based on tests by Williamson et al. (1994). Dominant and recessive alleles were detected after the digestion of the primary PCR product by the restriction enzyme TaqI. The identified PCR resistance marke
  • As model botanic species were selected tomato (Lycopersicon esculentum) and potato (Solanum tuberosum ssp. tuberosum). The potato was assessed for the collection of tetraploidic gene sources. The tomato was assessed for gene sources - varieties of the F1generation type and the segregating F2 generation. The potato's resistance against Ro1 of the Globodera rostochiensis pathotype was marked with a PCR amplification of the Gro 1.2.3 loci in chromosome II.. To assess the resistance, the results of experim ents conducted by Leister et al. (1997) were used. One loci of quantitative resistant - PCR marker has a close gene link with the monitored resistance. For the dominantly established tomato's resistance against Meloidogyne incognita was used a co-dominant CAPS marker of the Mi gene. The experiments were based on tests by Williamson et al. (1994). Dominant and recessive alleles were detected after the digestion of the primary PCR product by the restriction enzyme TaqI. The identified PCR resistance marke (en)
Title
  • Detection of Resistance Genes in Solanaceae Using DNA Markers
  • Detection of Resistance Genes in Solanaceae Using DNA Markers (en)
skos:prefLabel
  • Detection of Resistance Genes in Solanaceae Using DNA Markers
  • Detection of Resistance Genes in Solanaceae Using DNA Markers (en)
skos:notation
  • RIV/60460709:41210/01:00002739!RIV/2002/MSM/412102/N
http://linked.open.../vavai/riv/strany
  • 47
http://linked.open...avai/riv/aktivita
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  • Z(MSM 412100002)
http://linked.open...vai/riv/dodaniDat
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  • 677322
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  • RIV/60460709:41210/01:00002739
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http://linked.open.../riv/klicovaSlova
  • PCR, CAPS, DNA markers, Solanaceae, Globodera, Meloidogyne, tomato, Mi gen, (Lycopersicon esculentum), potato (Solanum tuberosum ssp. tuberosum), resistance, nematodes, PCN, MAS (en)
http://linked.open.../riv/klicoveSlovo
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  • [E43B1EA9C3CD]
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  • Třeboň
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  • České Budějovice
http://linked.open...i/riv/nazevZdroje
  • 4th International Symposium in the Series Recent Advances in Plant Biotechnology, Plant Molecular Biology for the New Millennium, Book of Abstracts
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  • Domkářová, J.
  • Kreuz, L.
  • Sedlák, Petr
  • Vejl, Pavel
  • Zouhar, Miloslav
  • Křenek, P.
  • Skupinová, Sylva
http://linked.open...vavai/riv/typAkce
http://linked.open.../riv/zahajeniAkce
http://linked.open...n/vavai/riv/zamer
number of pages
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  • Ústav molekulární biologie rostlin AV ČR
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  • 41210
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