About: Detection of novel auto-antigens in patients with recurrent miscarriage: description of an approach and preliminary findings

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About: Detection of novel auto-antigens in patients with recurrent miscarriage: description of an approach and preliminary findings Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4049207/
Description	Aim To develop and test a protocol for isolation of potential auto-antigens from chorionic tissue that may be linked to recurrent miscarriage (RM). Methods The strategy included: 1) isolation of IgGs tightly bound to chorionic tissue of RM patients by protein G chromatography; 2) construction of affinity columns using the chorionic antibodies for isolation of auto-antigens; 3) enrichment of auto-antigens from detergent extracted solution of chorionic proteins by affinity chromatography; 4) separation by dodecyl sulfate-electrophoresis followed by matrix-assisted laser desorption/ionization-time of flight mass spectrometry identification. Results Five potential auto-antigens were detected: neutral alpha-glucosidase AB, endoplasmin, transitional endoplasmic reticulum ATPase, putative endoplasmin-like protein, and cytoplasmic actin 2. Conclusions We developed a strategy for identification of auto-antigens in the chorionic tissue of women with RM, which could be of diagnostic and prognostic value. Aim To develop and test a protocol for isolation of potential auto-antigens from chorionic tissue that may be linked to recurrent miscarriage (RM). Methods The strategy included: 1) isolation of IgGs tightly bound to chorionic tissue of RM patients by protein G chromatography; 2) construction of affinity columns using the chorionic antibodies for isolation of auto-antigens; 3) enrichment of auto-antigens from detergent extracted solution of chorionic proteins by affinity chromatography; 4) separation by dodecyl sulfate-electrophoresis followed by matrix-assisted laser desorption/ionization-time of flight mass spectrometry identification. Results Five potential auto-antigens were detected: neutral alpha-glucosidase AB, endoplasmin, transitional endoplasmic reticulum ATPase, putative endoplasmin-like protein, and cytoplasmic actin 2. Conclusions We developed a strategy for identification of auto-antigens in the chorionic tissue of women with RM, which could be of diagnostic and prognostic value. (en)
Title	Detection of novel auto-antigens in patients with recurrent miscarriage: description of an approach and preliminary findings Detection of novel auto-antigens in patients with recurrent miscarriage: description of an approach and preliminary findings (en)
skos:prefLabel	Detection of novel auto-antigens in patients with recurrent miscarriage: description of an approach and preliminary findings Detection of novel auto-antigens in patients with recurrent miscarriage: description of an approach and preliminary findings (en)
skos:notation	RIV/60162694:G44__/14:43875157!RIV15-MO0-G44_____
http://linked.open...avai/riv/aktivita	N
http://linked.open...avai/riv/aktivity	N
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Lenčo, Juraj Vajrychová, Marie
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ministerstvo obrany / Univerzita obrany - Fakulta vojenského zdravotnictví Hradec Králové
http://linked.open...dnocenehoVysledku	10627
http://linked.open...ai/riv/idVysledku	RIV/60162694:G44__/14:43875157
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	beta; women; disease; proteins; gp96; autoantibodies; pregnancy loss (en)
http://linked.open.../riv/klicoveSlovo	autoantibodies disease women pregnancy loss beta proteins gp96
http://linked.open...odStatuVydavatele	HR - Chorvatská republika
http://linked.open...ontrolniKodProRIV	[1053C3BDCB6B]
http://linked.open...i/riv/nazevZdroje	Croatian Medical Journal
http://linked.open...in/vavai/riv/obor	FE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	55
http://linked.open...iv/tvurceVysledku	Lenčo, Juraj Vajrychová, Marie Kit, Yuriy Starykovych, Marina Stoika, Rostyslav Zastavna, Danuta
http://linked.open...ain/vavai/riv/wos	000338631600010
issn	0353-9504
number of pages	6 (xsd:int)
http://bibframe.org/vocab/doi	10.3325/cmj.2014.55.259
http://localhost/t...ganizacniJednotka	G44

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