About: Procedure for improved cleaning of FACSAria cuvette flow cell

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://onlinelibrary.wiley.com/doi/10.1002/cyto.a.22293/pdf
Description	Precision and reproducibility of FACS analyses carried out in the cuvette flow cell are affected by quality of the cuvette itself which might be significantly affected by contamination coming from samples. It can increase background fluorescence and cause light scattering, resulting in higher CV and lower precision. Using Hoechst 33342 and propidium iodide staining in our experiments on a BD FACSAria instrument, we encountered increasing Bright Bead %Robust CV (%rCV), especially in channels related to UV or violet lasers, and problems passing the CS&T procedure. Long-lasting cleaning procedures led only to temporary suppression of %rCVs and affected the lab workflow. We presumed that turbulent convection within the flow cell should produce more effective washing in comparison to linear flow. Here we present a simple and effective procedure that maintains the condition of the BD FACSAria cuvette flow cell and can be easily executed on a daily basis. Precision and reproducibility of FACS analyses carried out in the cuvette flow cell are affected by quality of the cuvette itself which might be significantly affected by contamination coming from samples. It can increase background fluorescence and cause light scattering, resulting in higher CV and lower precision. Using Hoechst 33342 and propidium iodide staining in our experiments on a BD FACSAria instrument, we encountered increasing Bright Bead %Robust CV (%rCV), especially in channels related to UV or violet lasers, and problems passing the CS&T procedure. Long-lasting cleaning procedures led only to temporary suppression of %rCVs and affected the lab workflow. We presumed that turbulent convection within the flow cell should produce more effective washing in comparison to linear flow. Here we present a simple and effective procedure that maintains the condition of the BD FACSAria cuvette flow cell and can be easily executed on a daily basis. (en)
Title	Procedure for improved cleaning of FACSAria cuvette flow cell Procedure for improved cleaning of FACSAria cuvette flow cell (en)
skos:prefLabel	Procedure for improved cleaning of FACSAria cuvette flow cell Procedure for improved cleaning of FACSAria cuvette flow cell (en)
skos:notation	RIV/60162694:G44__/13:43875027!RIV14-MO0-G44_____
http://linked.open...avai/riv/aktivita	V
http://linked.open...avai/riv/aktivity	V
http://linked.open...iv/cisloPeriodika	6
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Šinkorová, Zuzana
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ministerstvo obrany / Univerzita obrany - Fakulta vojenského zdravotnictví Hradec Králové
http://linked.open...dnocenehoVysledku	99893
http://linked.open...ai/riv/idVysledku	RIV/60162694:G44__/13:43875027
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	sodium dodecyl sulphate; turbulent convection; Contrad 70; propidium iodide; Hoechst 33342; FACSAria; cleaning; cuvette; flow cell (en)
http://linked.open.../riv/klicoveSlovo	Contrad 70 FACSAria Hoechst 33342 sodium dodecyl sulphate turbulent convection cuvette propidium iodide flow cell cleaning
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[C58336AD4898]
http://linked.open...i/riv/nazevZdroje	Cytometry Part A
http://linked.open...in/vavai/riv/obor	EC
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	83A
http://linked.open...iv/tvurceVysledku	Šinkorová, Zuzana Fedoročko, Peter Jendželovský, Rastislav Koval, Jan Mikeš, Jaromír Mikešová, Lucia
http://linked.open...ain/vavai/riv/wos	000319348900003
issn	1552-4922
number of pages	5 (xsd:int)
http://bibframe.org/vocab/doi	10.1002/cyto.a.22293
http://localhost/t...ganizacniJednotka	G44

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