About: Spectrophotometric assay of aflatoxin B1 using acetylcholinesterase immobilized on standard microplates

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About: Spectrophotometric assay of aflatoxin B1 using acetylcholinesterase immobilized on standard microplates Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://www.researchgate.net/journal/0172-780X_Neuro_endocrinology_letters
Description	Aflatoxins are a group of hepatotoxic secondary metabolites produced by molds Aspergillus. During inappropriate storage or processing of food and beverages, aflatoxins can be distributed and become a serious health problem. Disparate analytical techniques were prepared for the assay in the past. Here, a method based on inhibition of enzyme acetylcholinesterase (AChE) is performed allowing fast and simple prove of aflatoxins presence. For the assay purposes, AChE was immobilized on standard polystyrene microplates using gelatin as a stabilizing substance. Both free and immobilized AChEs were used and compared one to each other. Immobilization protocol was optimized and interference of organic solvents such as methanol was tested. For the immobilized AChE, limit of detection 2.75 ppb for aflatoxin B1 was received. The immobilized AChE kept full activity at least one month. Suitability of the assay for a practical performance is considered. Aflatoxins are a group of hepatotoxic secondary metabolites produced by molds Aspergillus. During inappropriate storage or processing of food and beverages, aflatoxins can be distributed and become a serious health problem. Disparate analytical techniques were prepared for the assay in the past. Here, a method based on inhibition of enzyme acetylcholinesterase (AChE) is performed allowing fast and simple prove of aflatoxins presence. For the assay purposes, AChE was immobilized on standard polystyrene microplates using gelatin as a stabilizing substance. Both free and immobilized AChEs were used and compared one to each other. Immobilization protocol was optimized and interference of organic solvents such as methanol was tested. For the immobilized AChE, limit of detection 2.75 ppb for aflatoxin B1 was received. The immobilized AChE kept full activity at least one month. Suitability of the assay for a practical performance is considered. (en)
Title	Spectrophotometric assay of aflatoxin B1 using acetylcholinesterase immobilized on standard microplates Spectrophotometric assay of aflatoxin B1 using acetylcholinesterase immobilized on standard microplates (en)
skos:prefLabel	Spectrophotometric assay of aflatoxin B1 using acetylcholinesterase immobilized on standard microplates Spectrophotometric assay of aflatoxin B1 using acetylcholinesterase immobilized on standard microplates (en)
skos:notation	RIV/60162694:G44__/13:43874952!RIV14-MO0-G44_____
http://linked.open...avai/riv/aktivita	I
http://linked.open...avai/riv/aktivity	I
http://linked.open...iv/cisloPeriodika	8
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Pohanka, Doc. RNDr. PhD, Miroslav
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ministerstvo obrany / Univerzita obrany - Fakulta vojenského zdravotnictví Hradec Králové
http://linked.open...dnocenehoVysledku	106996
http://linked.open...ai/riv/idVysledku	RIV/60162694:G44__/13:43874952
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Mycotoxin; Ellman method; Butyrylcholinesterase; Biosensor; Aspergillus; Aflatoxin; Acetylcholinesterase (en)
http://linked.open.../riv/klicoveSlovo	Mycotoxin Acetylcholinesterase Aspergillus Aflatoxin Ellman method Biosensor Butyrylcholinesterase
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[42D7F1D61256]
http://linked.open...i/riv/nazevZdroje	Analytical Letters
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	1 (xsd:int)
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	46
http://linked.open...iv/tvurceVysledku	Pohanka, Miroslav
http://linked.open...ain/vavai/riv/wos	000318281700011
issn	0003-2719
number of pages	10 (xsd:int)
http://bibframe.org/vocab/doi	10.1080/00032719.2012.757703
http://localhost/t...ganizacniJednotka	G44

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