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| Description
| - The establishment of protoplast cultures of Allium species was tested as the prerequisite for somatic hybridization as a tool of increasing of genetic variability. Protoplasts were isolated from in vitro cultivated plants of bear's garlic - A. usinum, and ex vitro cloves of garlic – A. sativum. There were used two modified enzymatic solutions (Yamashita et al. 2002): 1% Cellulase Onozuka R-10, 0.5% Macerozyme R-10 and 0.5% Hemicellulase; 2% Cellulase Onozuka R-10, 0.5% Macerozyme R-10 and 1% Hemicellulase. The workflow of isolation was done according to protoco : http://www.vubhb.cz/_te.asp?F=institute/06dogr-protocols-mfpep.h m with washing solution by Yamashita et al. (2002). Kao and Michayluk cultivation medium (1975) was completed 0.2 M sucrose and 0.2 M glucose (Shimonaka et al. 2001) and 2 mM Putrescine dihydrochloride. The enzymatic solution of higher concentration was suitable both for A. ursinum and A. sativum with working time 21 hours and 9 hours respectively. The cultivated protoplasts maintained viability - application of Putrescine showed to be essential compare to control. Although there was observed cell walls regeneration and occasionally cell division, the major part of protoplasts kept spherical shape. Higher degrees of regeneration were not achieved.
- The establishment of protoplast cultures of Allium species was tested as the prerequisite for somatic hybridization as a tool of increasing of genetic variability. Protoplasts were isolated from in vitro cultivated plants of bear's garlic - A. usinum, and ex vitro cloves of garlic – A. sativum. There were used two modified enzymatic solutions (Yamashita et al. 2002): 1% Cellulase Onozuka R-10, 0.5% Macerozyme R-10 and 0.5% Hemicellulase; 2% Cellulase Onozuka R-10, 0.5% Macerozyme R-10 and 1% Hemicellulase. The workflow of isolation was done according to protoco : http://www.vubhb.cz/_te.asp?F=institute/06dogr-protocols-mfpep.h m with washing solution by Yamashita et al. (2002). Kao and Michayluk cultivation medium (1975) was completed 0.2 M sucrose and 0.2 M glucose (Shimonaka et al. 2001) and 2 mM Putrescine dihydrochloride. The enzymatic solution of higher concentration was suitable both for A. ursinum and A. sativum with working time 21 hours and 9 hours respectively. The cultivated protoplasts maintained viability - application of Putrescine showed to be essential compare to control. Although there was observed cell walls regeneration and occasionally cell division, the major part of protoplasts kept spherical shape. Higher degrees of regeneration were not achieved. (en)
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| Title
| - Introduction to protoplast culture of Allium ursinum and A. sativum
- Introduction to protoplast culture of Allium ursinum and A. sativum (en)
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| skos:prefLabel
| - Introduction to protoplast culture of Allium ursinum and A. sativum
- Introduction to protoplast culture of Allium ursinum and A. sativum (en)
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| skos:notation
| - RIV/60109807:_____/13:#0000226!RIV14-MSM-60109807
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| http://linked.open...avai/predkladatel
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/60109807:_____/13:#0000226
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - Allium; protoplast; putrescine (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...iv/tvurceVysledku
| - Domkářová, Jaroslava
- Greplová, Marie
- Polzerová, Hana
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