About: PRODUCTION OF POLYCLONAL ANTIBODIES AGAINST RECOMBINANT POTATO VIRUS X COAT PROTEIN

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The gene encoding the coat protein (CP) of Potato virus X was cloned into expression vector pMPM-4A Omega. PVX CP was expressed in Escherichia coli and was used for raising rabbit polyclonal antibodies. For immunization we purified CP fractions from bacterial lysate either by simple fractionation or by excision from SDS gel. The obtained sera and antibodies were tested for the detection of PVX laboratory hosts Nicotiana tabacum, cv. Petit Havana SR 1 and N.benthamiana and in various cultivars of natural host Solanum tuberosum by ELISA as well as by Western blots. The antibodies were specific for PVX and are usable for the detection of the broad isolates spectrum of the Potato virus X by ELISA and Western blot. The gene encoding the coat protein (CP) of Potato virus X was cloned into expression vector pMPM-4A Omega. PVX CP was expressed in Escherichia coli and was used for raising rabbit polyclonal antibodies. For immunization we purified CP fractions from bacterial lysate either by simple fractionation or by excision from SDS gel. The obtained sera and antibodies were tested for the detection of PVX laboratory hosts Nicotiana tabacum, cv. Petit Havana SR 1 and N.benthamiana and in various cultivars of natural host Solanum tuberosum by ELISA as well as by Western blots. The antibodies were specific for PVX and are usable for the detection of the broad isolates spectrum of the Potato virus X by ELISA and Western blot. (en)
Title	PRODUCTION OF POLYCLONAL ANTIBODIES AGAINST RECOMBINANT POTATO VIRUS X COAT PROTEIN PRODUCTION OF POLYCLONAL ANTIBODIES AGAINST RECOMBINANT POTATO VIRUS X COAT PROTEIN (en)
skos:prefLabel	PRODUCTION OF POLYCLONAL ANTIBODIES AGAINST RECOMBINANT POTATO VIRUS X COAT PROTEIN PRODUCTION OF POLYCLONAL ANTIBODIES AGAINST RECOMBINANT POTATO VIRUS X COAT PROTEIN (en)
skos:notation	RIV/60109807:_____/08:0R000676!RIV13-MSM-60109807
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(1M06030), Z(AV0Z50380511)
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Dědič, Petr
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav bramborářský Havlíčkův Brod, s.r.o.
http://linked.open...dnocenehoVysledku	390089
http://linked.open...ai/riv/idVysledku	RIV/60109807:_____/08:0R000676
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	PVX; recombinant antibodies; laboratory diagnozis (en)
http://linked.open.../riv/klicoveSlovo	PVX laboratory diagnozis recombinant antibodies
http://linked.open...ontrolniKodProRIV	[0C373BBB4C4D]
http://linked.open...v/mistoKonaniAkce	Křtiny
http://linked.open...i/riv/mistoVydani	Brno
http://linked.open...i/riv/nazevZdroje	Funkční genomika a proteomika ve šlechtění rostlin
http://linked.open...in/vavai/riv/obor	GF
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Functional genomics and proteomics for crop improvement
http://linked.open...UplatneniVysledku	2008
http://linked.open...iv/tvurceVysledku	Moravec, Tomáš Plchová, Helena Čeřovská, Noemi Dědič, Petr Filigarová, Marie
http://linked.open...vavai/riv/typAkce	CST - Celostátní
http://linked.open.../riv/zahajeniAkce	2008-09-09 (xsd:date)
http://linked.open...n/vavai/riv/zamer	Mechanismy regulace růstu a vývoje rostlin na úrovni buněk, orgánů a celých organismů: fyziologické, genetické a molekulárně biologické základy
number of pages	4 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Neuveden
https://schema.org/isbn	978-80-7375-234-7
is http://linked.open...avai/riv/vysledek of	PRODUCTION OF POLYCLONAL ANTIBODIES AGAINST RECOMBINANT POTATO VIRUS X COAT PROTEIN

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