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| Description
| - Weed plants characteristic for potato fields have not been considered in the past as potential hosts that could transmit and lead to spreading of potato spindle tuber viroid (PSTVd). To gain insight into this problem, we biolistically inoculated these weed plants with viroid populations either as RNA or as cDNA PSTVd variants. New potential viroid host species, collected in central Europe, were discovered. From 12 weed species characteristic for potato fields, high viroid levels, detectable by molecular hybridization, were maintained after both RNA and DNA transfers in Chamomilla reculita and Anthemis arvensis. Low viroid levels, detectable by reverse transcription-PCR (RT-PCR) only, were maintained after plant inoculations with cDNA in Veronica argensis and Amaranthus retroflexus. In cultured chamomille (Chamomilla reculita var.Bohemia) the pathogenesis symptoms were observed in similar strength as for tomato cv. Rutgers. The strongest symptoms were characteristic for letal strain AS1.
- Weed plants characteristic for potato fields have not been considered in the past as potential hosts that could transmit and lead to spreading of potato spindle tuber viroid (PSTVd). To gain insight into this problem, we biolistically inoculated these weed plants with viroid populations either as RNA or as cDNA PSTVd variants. New potential viroid host species, collected in central Europe, were discovered. From 12 weed species characteristic for potato fields, high viroid levels, detectable by molecular hybridization, were maintained after both RNA and DNA transfers in Chamomilla reculita and Anthemis arvensis. Low viroid levels, detectable by reverse transcription-PCR (RT-PCR) only, were maintained after plant inoculations with cDNA in Veronica argensis and Amaranthus retroflexus. In cultured chamomille (Chamomilla reculita var.Bohemia) the pathogenesis symptoms were observed in similar strength as for tomato cv. Rutgers. The strongest symptoms were characteristic for letal strain AS1. (en)
- Plevelné rostliny běžně se vyskytující na bramborových polích, nebyly doposud pokládány za hostitelské rostliny, tj. rostliny umožňující přenos PSTVd na brambory. Biolisticky jsme inokulovali vybrané plevelné rostliny populacemi viroidů, jak RNA tak cDNA variantami PSTVd. Byli nalezeni noví potenciální hostitelé PSTVd z oblasti střední Evropy. Z 12 druhů plevelných rostlin přítomných na bramborových polích, vysoké hladiny PSTVd, detekovatelné i molekulární hybridizací, byly udržovány v rostlinách Chammomilla reculita a Anthemis arvensis po RNA i cDNA přenosu PSTVd. Nízká hladina viroidu, detekovatelná pouze RT-PCR, byla udržována ve Veronica argensis a Amarantus retroflexus. po inokulaci rostlin cDNA PSTVd. V těchto dvou plevelích byla koncentrace 105 event. 103 krát nižší než v rajčatech (zjištěno pomocí kvantitativní RT-PCR). Nejsilnější symptomy a největší zmenšení růstu byly charakteristické pro letální kmen AS1, „mírné“ varianty virodu nezpůsobovaly tak zřetelné symptomy. (cs)
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| Title
| - Biolistic Transmission of potato spindle tuber viroid (PSTVd) Populations to Weeds frequently grown on Potato Fields and PSTVd pathogenesis on Cultured Chamomilla reculita
- Biolistický přenos populací viroidu vřetenovitosti hlíz bramboru (PSTVd) do plevelných rostlin vyskytujících se na bramborovýc polích a patogeneze PSTVd na Chamomilla reculita (cs)
- Biolistic Transmission of potato spindle tuber viroid (PSTVd) Populations to Weeds frequently grown on Potato Fields and PSTVd pathogenesis on Cultured Chamomilla reculita (en)
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| skos:prefLabel
| - Biolistic Transmission of potato spindle tuber viroid (PSTVd) Populations to Weeds frequently grown on Potato Fields and PSTVd pathogenesis on Cultured Chamomilla reculita
- Biolistický přenos populací viroidu vřetenovitosti hlíz bramboru (PSTVd) do plevelných rostlin vyskytujících se na bramborovýc polích a patogeneze PSTVd na Chamomilla reculita (cs)
- Biolistic Transmission of potato spindle tuber viroid (PSTVd) Populations to Weeds frequently grown on Potato Fields and PSTVd pathogenesis on Cultured Chamomilla reculita (en)
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| skos:notation
| - RIV/60109807:_____/08:#0000099!RIV09-MSM-60109807
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/60109807:_____/08:#0000099
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - potato fields, weed plants, potato spindle tuber viroid (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
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| http://linked.open...n/vavai/riv/zamer
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| number of pages
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| is http://linked.open...avai/riv/vysledek
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