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Description
  • Byl vyvinut nový extrakční postup pro detekci PVY a PVS pomocí dot-blot hybridizace. Zlepšený extrakční pufr obsahující 5% SDS umožňoval citlivou detekci bez potřeby purifikovat RNA. Pro PVS byly vyvinuta alternativní metoda biolistické inokulace, pomocí které bylo dosahováno silného patogenního účinku na rajčatech. (cs)
  • New extraction procedure was developed to detect potato virus Y and S by a dot-blot hybridization. Improved extraction buffer that contains 5% SDS enabled a sensitive detection without need to purify RNA. An alternative biolostic inoculation method was developed for potato virus S (PVS) which led to strong pathogenic effect on tomato.
  • New extraction procedure was developed to detect potato virus Y and S by a dot-blot hybridization. Improved extraction buffer that contains 5% SDS enabled a sensitive detection without need to purify RNA. An alternative biolostic inoculation method was developed for potato virus S (PVS) which led to strong pathogenic effect on tomato. (en)
Title
  • Biolistic inoculation and improved detection of PVS by molecular hybridization
  • Biolistic inoculation and improved detection of PVS by molecular hybridization (en)
  • Biolostická inokulace a zlepšená detekce PVS pomocí molekulární hybridizace (cs)
skos:prefLabel
  • Biolistic inoculation and improved detection of PVS by molecular hybridization
  • Biolistic inoculation and improved detection of PVS by molecular hybridization (en)
  • Biolostická inokulace a zlepšená detekce PVS pomocí molekulární hybridizace (cs)
skos:notation
  • RIV/60109807:_____/06:9P000919!RIV09-MZE-60109807
http://linked.open...avai/riv/aktivita
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  • P(QF3109)
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  • 467020
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  • RIV/60109807:_____/06:9P000919
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  • potato; PVS; PVY; molecular hzbridiyation; biolostic inoculation (en)
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  • [518CA0469A96]
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  • Praha
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  • Praha
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  • Sborník příspěvků XVII. česká a slovenská konference o ochraně rostlin
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  • Matoušek, J.
  • Dědič, Petr
  • Schubert, J.
  • Kozlová, P.
  • Růžková, E.
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http://linked.open.../riv/zahajeniAkce
number of pages
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  • ÚVTI-Z
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  • 80-213-1564-4
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