About: The use of TALENs for nonhomologous end joining mutagenesis in silkworm and fruitly

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About: The use of TALENs for nonhomologous end joining mutagenesis in silkworm and fruitly Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://www.sciencedirect.com/science/article/pii/S1046202314000449
Description	Transcription activator-like effector nucleases (TALENs) are custom-made enzymes designed to cut double-stranded DNA at desired locations. The DNA breaks are repaired either by error-prone non-homologous end-joining (NHEJ) pathway or via homologous recombination requiring homologous DNA as a template for the repair. TALENs are used for site-specific mutagenesis in an extended range of organisms including insects. We will describe here a simple TALEN-based mutagenesis protocol suitable for the generation of germline mutations in Bombyx mori and Drosophila melanogaster. The protocol includes assembly of specific TAL modules, in vitro synthesis of TALEN RNAs, egg microinjection and mutation detection using PCR analysis. Our procedure allows a high frequency induction of NHEJ mutations, which often allows the reception of homozygous mutants already in the G1. Transcription activator-like effector nucleases (TALENs) are custom-made enzymes designed to cut double-stranded DNA at desired locations. The DNA breaks are repaired either by error-prone non-homologous end-joining (NHEJ) pathway or via homologous recombination requiring homologous DNA as a template for the repair. TALENs are used for site-specific mutagenesis in an extended range of organisms including insects. We will describe here a simple TALEN-based mutagenesis protocol suitable for the generation of germline mutations in Bombyx mori and Drosophila melanogaster. The protocol includes assembly of specific TAL modules, in vitro synthesis of TALEN RNAs, egg microinjection and mutation detection using PCR analysis. Our procedure allows a high frequency induction of NHEJ mutations, which often allows the reception of homozygous mutants already in the G1. (en)
Title	The use of TALENs for nonhomologous end joining mutagenesis in silkworm and fruitly The use of TALENs for nonhomologous end joining mutagenesis in silkworm and fruitly (en)
skos:prefLabel	The use of TALENs for nonhomologous end joining mutagenesis in silkworm and fruitly The use of TALENs for nonhomologous end joining mutagenesis in silkworm and fruitly (en)
skos:notation	RIV/60077344:_____/14:00426076!RIV15-GA0-60077344
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(GAP305/10/2406)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Žurovec, Michal Sajwan, Suresh
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biologické centrum AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	52361
http://linked.open...ai/riv/idVysledku	RIV/60077344:_____/14:00426076
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Bombyx mori; Drosophila melanogaster; pBlue-TAL (en)
http://linked.open.../riv/klicoveSlovo	Drosophila melanogaster Bombyx mori pBlue-TAL
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[29D6A3177041]
http://linked.open...i/riv/nazevZdroje	Methods
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Targeted mutagenesis in silkworm
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	69
http://linked.open...iv/tvurceVysledku	Žurovec, Michal Sajwan, Suresh Takasu, Y. Tamura, T. Kobayashi, I.
http://linked.open...ain/vavai/riv/wos	000341803500008
issn	1046-2023
number of pages	12 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.ymeth.2014.02.014

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