About: Over-Expression of the Transcription Factor Hlmyb3 in Transgenic Hop (Humulus lupulus L.): Influence on the Expression of Genes Involved in the Biosynthesis of Flavonoids and Phloroglucinols

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About: Over-Expression of the Transcription Factor Hlmyb3 in Transgenic Hop (Humulus lupulus L.): Influence on the Expression of Genes Involved in the Biosynthesis of Flavonoids and Phloroglucinols Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://www.actahort.org/books/1010/
Description	In hop (Humulus lupulus L.), prenylated chalcones, like xanthohumol and desmethylxanthohumol, have received considerable attention due to their anti-cancer properties. However, their concentration in wildtype plants is rather low. To enhance their production, it would be interesting to modify the regulation of genes involved in the biosynthesis of flavonoids. Towards this goal, the endogenous regulatory gene hlmyb3 was genetically introduced into hop cultivar Tettnanger’ by Agrobacterium-mediated genetic transformation. The endogenous hlmyb3 was stably incorporated and expressed in the hop genome. By quantitative real-time PCR, it was demonstrated that hlmyb3 was highly expressed in transgenic plants compared to wildtype plants. Moreover, transgenic plants with elevated expression of chs_h1, chi, f3’h, vps, and omt1 were identified. In hop (Humulus lupulus L.), prenylated chalcones, like xanthohumol and desmethylxanthohumol, have received considerable attention due to their anti-cancer properties. However, their concentration in wildtype plants is rather low. To enhance their production, it would be interesting to modify the regulation of genes involved in the biosynthesis of flavonoids. Towards this goal, the endogenous regulatory gene hlmyb3 was genetically introduced into hop cultivar Tettnanger’ by Agrobacterium-mediated genetic transformation. The endogenous hlmyb3 was stably incorporated and expressed in the hop genome. By quantitative real-time PCR, it was demonstrated that hlmyb3 was highly expressed in transgenic plants compared to wildtype plants. Moreover, transgenic plants with elevated expression of chs_h1, chi, f3’h, vps, and omt1 were identified. (en)
Title	Over-Expression of the Transcription Factor Hlmyb3 in Transgenic Hop (Humulus lupulus L.): Influence on the Expression of Genes Involved in the Biosynthesis of Flavonoids and Phloroglucinols Over-Expression of the Transcription Factor Hlmyb3 in Transgenic Hop (Humulus lupulus L.): Influence on the Expression of Genes Involved in the Biosynthesis of Flavonoids and Phloroglucinols (en)
skos:prefLabel	Over-Expression of the Transcription Factor Hlmyb3 in Transgenic Hop (Humulus lupulus L.): Influence on the Expression of Genes Involved in the Biosynthesis of Flavonoids and Phloroglucinols Over-Expression of the Transcription Factor Hlmyb3 in Transgenic Hop (Humulus lupulus L.): Influence on the Expression of Genes Involved in the Biosynthesis of Flavonoids and Phloroglucinols (en)
skos:notation	RIV/60077344:_____/13:00426214!RIV14-AV0-60077344
http://linked.open...avai/predkladatel	Biologické centrum AV ČR, v. v. i.
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(GA13-03037S), P(QH81052)
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Matoušek, Jaroslav
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biologické centrum AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	95009
http://linked.open...ai/riv/idVysledku	RIV/60077344:_____/13:00426214
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	MYB transcription factors; genetic transformation; gene expression; quantitative real-time PCR (en)
http://linked.open.../riv/klicoveSlovo	quantitative real-time PCR MYB transcription factors gene expression genetic transformation
http://linked.open...ontrolniKodProRIV	[81EAB5BE96BA]
http://linked.open...v/mistoKonaniAkce	Žatec
http://linked.open...i/riv/mistoVydani	Leuven
http://linked.open...i/riv/nazevZdroje	Proceedings of the International Humulus Symposium /3./
http://linked.open...in/vavai/riv/obor	GE
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Combinatorial regulation and network of transcription factors involved in the biosynthesis of medicinal prenylflavonoids in hop (Humulus lupulus L.). Development of molecular-genetic markers for modern breeding and gene engineering of hop (Humulus lupulus) based on the system of genomic and expression libraries.
http://linked.open...UplatneniVysledku	2013
http://linked.open...iv/tvurceVysledku	Matoušek, Jaroslav Weber, G. Gatica-Arias, A. Stanke, M. Häntzschel, K. R.
http://linked.open...vavai/riv/typAkce	WRD - Světová
http://linked.open.../riv/zahajeniAkce	2012-09-09 (xsd:date)
number of pages	5 (xsd:int)
http://purl.org/ne...btex#hasPublisher	ISHS Acta Horticulturae
https://schema.org/isbn	9789066056961

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