About: Development of a Multilocus Sequence Tool for Typing Cryptosporidium muris and Cryptosporidium andersoni

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About: Development of a Multilocus Sequence Tool for Typing Cryptosporidium muris and Cryptosporidium andersoni Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Although widely used for the characterization of the transmission of intestinal Cryptosporidium spp., genotyping tools are not available for C. muris and C. andersoni, 2 of the most common gastric spp. infecting mammals. In this study, we screened the C. muris whole-genome sequencing data - among the 13 potential loci (6 microsatellite and 7 minisatellite loci) evaluated by PCR and DNA sequencing, 4 were chosen. DNA sequence analyses of 44 samples showed the presence of 5 to 10 subtypes of C. muris and 1 to 4 subtypes of C. andersoni at each locus. Altogether, 11 C. muris and 7 C. andersoni multilocus sequence typing (MLST) subtypes were detected among the 16 C. muris and 12 C. andersoni specimens successfully sequenced at all 4 loci. In all analyses, the C. muris isolate (TS03) that originated from an East African mole rat differed significantly from other C. muris isolates, approaching the extent of genetic differences between C. muris and C. andersoni. Although widely used for the characterization of the transmission of intestinal Cryptosporidium spp., genotyping tools are not available for C. muris and C. andersoni, 2 of the most common gastric spp. infecting mammals. In this study, we screened the C. muris whole-genome sequencing data - among the 13 potential loci (6 microsatellite and 7 minisatellite loci) evaluated by PCR and DNA sequencing, 4 were chosen. DNA sequence analyses of 44 samples showed the presence of 5 to 10 subtypes of C. muris and 1 to 4 subtypes of C. andersoni at each locus. Altogether, 11 C. muris and 7 C. andersoni multilocus sequence typing (MLST) subtypes were detected among the 16 C. muris and 12 C. andersoni specimens successfully sequenced at all 4 loci. In all analyses, the C. muris isolate (TS03) that originated from an East African mole rat differed significantly from other C. muris isolates, approaching the extent of genetic differences between C. muris and C. andersoni. (en)
Title	Development of a Multilocus Sequence Tool for Typing Cryptosporidium muris and Cryptosporidium andersoni Development of a Multilocus Sequence Tool for Typing Cryptosporidium muris and Cryptosporidium andersoni (en)
skos:prefLabel	Development of a Multilocus Sequence Tool for Typing Cryptosporidium muris and Cryptosporidium andersoni Development of a Multilocus Sequence Tool for Typing Cryptosporidium muris and Cryptosporidium andersoni (en)
skos:notation	RIV/60077344:_____/11:00358458!RIV12-AV0-60077344
http://linked.open...avai/predkladatel	Biologické centrum AV ČR, v. v. i.
http://linked.open...avai/riv/aktivita	N Z
http://linked.open...avai/riv/aktivity	N, Z(AV0Z60220518)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Modrý, David Kváč, Martin Koudela, Břetislav
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biologické centrum AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	194019
http://linked.open...ai/riv/idVysledku	RIV/60077344:_____/11:00358458
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	ASTERN UNITED-STATES; RIBOSOMAL-RNA GENE; PHYLOGENETIC ANALYSIS; MOLECULAR ANALYSIS; NATURAL INFECTION; DAIRY-CATTLE; PREVALENCE; GENOTYPES; HUMANS; KENYA (en)
http://linked.open.../riv/klicoveSlovo	ASTERN UNITED-STATES DAIRY-CATTLE GENOTYPES HUMANS KENYA MOLECULAR ANALYSIS PREVALENCE RIBOSOMAL-RNA GENE PHYLOGENETIC ANALYSIS NATURAL INFECTION
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[7A02B1B203D7]
http://linked.open...i/riv/nazevZdroje	Journal of Clinical Microbiology
http://linked.open...in/vavai/riv/obor	GJ
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	10 (xsd:int)
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	49
http://linked.open...iv/tvurceVysledku	Kváč, Martin Li, N. Modrý, David Fayer, R. Feng, Y. Koudela, Břetislav Ryan, U. M. Xiao, L. Yang, W. Zhang, L.
http://linked.open...ain/vavai/riv/wos	000285787100003
http://linked.open...n/vavai/riv/zamer	Parazitismus a parazito-hostitelské vztahy na organismální, buněčné a molekulové úrovni
issn	0095-1137
number of pages	8 (xsd:int)
http://bibframe.org/vocab/doi	10.1128/JCM.01329-10
is http://linked.open...avai/riv/vysledek of	Development of a Multilocus Sequence Tool for Typing Cryptosporidium muris and Cryptosporidium andersoni Development of a Multilocus Sequence Tool for Typing Cryptosporidium muris and Cryptosporidium andersoni

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