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| rdf:type
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| Description
| - Spirocerca lupi (Spirurida) is a life-threatening parasitic nematode of dogs that is presently emerging in several countries. Given the significant benefit of improved diagnosis, the present work evaluated the reliability of a recently described copromicroscopic approach, the FLOTAC technique, as well as a PCR-based assay with that of traditional coproscopic techniques to diagnose S. lupi infection. The coproscopic examinations showed low sensitivity and high variability, demonstrating the unreliability of the conventional methods for detecting S. lupi eggs. FLOTAC technique scored the highest number of positives and significantly higher number of S. lupi eggs per microscopic field compared to the other coproscopic methods. Additionally, of the coproscopically negative samples, 9 (45%) Kenyan and 21 (38.2%) Israeli samples scored molecularly positive using the PCR-based approach. The potential implications and perspectives for canine spirocercosis of these methodologies are discussed.
- Spirocerca lupi (Spirurida) is a life-threatening parasitic nematode of dogs that is presently emerging in several countries. Given the significant benefit of improved diagnosis, the present work evaluated the reliability of a recently described copromicroscopic approach, the FLOTAC technique, as well as a PCR-based assay with that of traditional coproscopic techniques to diagnose S. lupi infection. The coproscopic examinations showed low sensitivity and high variability, demonstrating the unreliability of the conventional methods for detecting S. lupi eggs. FLOTAC technique scored the highest number of positives and significantly higher number of S. lupi eggs per microscopic field compared to the other coproscopic methods. Additionally, of the coproscopically negative samples, 9 (45%) Kenyan and 21 (38.2%) Israeli samples scored molecularly positive using the PCR-based approach. The potential implications and perspectives for canine spirocercosis of these methodologies are discussed. (en)
- Spirocerca lupi (Spirurida) je život ohrožující parazitický nematod psů s rostoucím významem. Předložená práce hodnotí recentně popsaný kopromiskroskopický přístup v diagnostice (FLOTAC) a diagnostiku založenou na PCR Koproskopické vyšetření vykazovalo nízkou citlivost a vysokou variabilitu. Technika FLOTAC odhalila nejvyšší počet pozitivních vzorků ve srovnání s ostatními mikroskopickými metodami. Navíc, 9 (45%) keňských a 21 (38.2%) izraelských vzorků bylo vyhodnoceno jako pozitivní s využitím PCR. Diskutováno je potenciální využití sledovaných metod pro diagnostiku psí spirocerkózy. (cs)
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| Title
| - Copromicroscopic and molecular assays for the detection of cancer-causing parasitic nematode Spirocerca lupi
- Kopromikroskopická a molekulární diagnostika nematoda Spirocerca lupi (cs)
- Copromicroscopic and molecular assays for the detection of cancer-causing parasitic nematode Spirocerca lupi (en)
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| skos:prefLabel
| - Copromicroscopic and molecular assays for the detection of cancer-causing parasitic nematode Spirocerca lupi
- Kopromikroskopická a molekulární diagnostika nematoda Spirocerca lupi (cs)
- Copromicroscopic and molecular assays for the detection of cancer-causing parasitic nematode Spirocerca lupi (en)
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| skos:notation
| - RIV/60077344:_____/08:00321788!RIV09-AV0-60077344
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/60077344:_____/08:00321788
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - spirocercosis; dog; coproscopy; FLOTAC; mitochondrial DNA; cox1 gene; diagnosis (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Modrý, David
- Albrechtová, K.
- Mihalca, A. D.
- Aroch, I.
- Avolio, S.
- Cringoli, G.
- Iorio, R.
- Lavy, E.
- Milillo, P.
- Otranto, D.
- Traversa, D.
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| http://linked.open...ain/vavai/riv/wos
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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| is http://linked.open...avai/riv/vysledek
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