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  • Glycosylation of Dorin M, protein isolated from soft tick Ornithodors moubata, was fully characterized by means of liquid chromatography coupled to tandem mass spectrometry. Protein was found to be N-glycosylated on three sites. Two bore high-mannose structures while the third one was modified by truncated, core fucosylated, mannose glycan. Besides the types of glycan, the heterogeneity was also estimated based on the retention behavior of individual glycopeptides
  • Glycosylation of Dorin M, protein isolated from soft tick Ornithodors moubata, was fully characterized by means of liquid chromatography coupled to tandem mass spectrometry. Protein was found to be N-glycosylated on three sites. Two bore high-mannose structures while the third one was modified by truncated, core fucosylated, mannose glycan. Besides the types of glycan, the heterogeneity was also estimated based on the retention behavior of individual glycopeptides (en)
  • Kapalinová chromatografie napojená na tandemovou hmotnostní spektrometrii byla použita k detailní charakterizaci glykosylace Dorinu M, proteinu izolovaného z klíštěte Ornithodors moubata. Na protein byla nalezena tři místa N-glykosylace. Dvě byla modifikována vysokomanosovými strukturami a třetí neslo zkrácenou variantu manosvé struktury fukosylovanou na prvním N-acetylglukosaminu. Kromě detailního popisu typu sacharidových struktur byla na základě chromatografického chování jednotlivých glykopeptidů určena i mikroheterogenita na jednotlivých glykosylačních místech (cs)
Title
  • Deciphering Dorin M glycosylation by mass spectrometry
  • Deciphering Dorin M glycosylation by mass spectrometry (en)
  • Určení glykosylace Dorinu M hmotnostní spektrometií (cs)
skos:prefLabel
  • Deciphering Dorin M glycosylation by mass spectrometry
  • Deciphering Dorin M glycosylation by mass spectrometry (en)
  • Určení glykosylace Dorinu M hmotnostní spektrometií (cs)
skos:notation
  • RIV/60077344:_____/08:00320700!RIV09-GA0-60077344
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GD524/03/H133), P(LC06009), P(LC545), S, Z(AV0Z50200510), Z(AV0Z60220518), Z(MSM6007665801)
http://linked.open...iv/cisloPeriodika
  • 6
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 362267
http://linked.open...ai/riv/idVysledku
  • RIV/60077344:_____/08:00320700
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • glycosylation; tandem mass spectrometry; lectin (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV
  • [10293DBAEF6F]
http://linked.open...i/riv/nazevZdroje
  • European Journal of Mass Spectrometry
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 14
http://linked.open...iv/tvurceVysledku
  • Grubhoffer, Libor
  • Kavan, Daniel
  • Kopáček, Petr
  • Kovář, Vojtěch
  • Man, Petr
  • Havlíček, Vladimír
  • Štěrba, Ján
  • Strohalm, Martin
http://linked.open...ain/vavai/riv/wos
  • 000262813100003
http://linked.open...n/vavai/riv/zamer
issn
  • 1469-0667
number of pages
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