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  • Characterization of entomogenous and mycoparasitic fungi using RAPD technique with special attention to evaluate genetic stability of strains that are used as the active ingredients in commercial biopesticides was subject of the study. Strain specific fingerprints were constructed for Paecilomyces fumosoroseus - strain PFR 97 Apopka, Gliocladium virens - strain GL 21 and Verticillium lecanii strain MYCOTAL. Genetic stability and homogeneity was confirmed among re-isolates that were obtained from commercial batches of bio-insecticide PFR 97TM20%G and bio-fungicide SoilGard# that were produced within 1995-1999. RAPD analysis indicates the genetic identity among V. lecanii strains re-isolated from two different bio-insecticides - MYCOTAL# & VERTALEC#. Theapplied utility of RAPD technique was demonstrated when P. fumosoroseus strain PFR 97-Apopka was reliably identified after passed through adults of spruce bark beetle Ips typographus, and in case study, where RAPD analysis was used to evaluate relations
  • Characterization of entomogenous and mycoparasitic fungi using RAPD technique with special attention to evaluate genetic stability of strains that are used as the active ingredients in commercial biopesticides was subject of the study. Strain specific fingerprints were constructed for Paecilomyces fumosoroseus - strain PFR 97 Apopka, Gliocladium virens - strain GL 21 and Verticillium lecanii strain MYCOTAL. Genetic stability and homogeneity was confirmed among re-isolates that were obtained from commercial batches of bio-insecticide PFR 97TM20%G and bio-fungicide SoilGard# that were produced within 1995-1999. RAPD analysis indicates the genetic identity among V. lecanii strains re-isolated from two different bio-insecticides - MYCOTAL# & VERTALEC#. Theapplied utility of RAPD technique was demonstrated when P. fumosoroseus strain PFR 97-Apopka was reliably identified after passed through adults of spruce bark beetle Ips typographus, and in case study, where RAPD analysis was used to evaluate relations (en)
Title
  • Characterization and identification of entomopathogenic and mycoparasitic fungi using RAPD-PCR technique
  • Characterization and identification of entomopathogenic and mycoparasitic fungi using RAPD-PCR technique (en)
skos:prefLabel
  • Characterization and identification of entomopathogenic and mycoparasitic fungi using RAPD-PCR technique
  • Characterization and identification of entomopathogenic and mycoparasitic fungi using RAPD-PCR technique (en)
skos:notation
  • RIV/60076658:_____/02:00004048!RIV/2003/MSM/120003/N
http://linked.open.../vavai/riv/strany
  • 1-12
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  • Z(MSM 122200002)
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  • 1
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  • 640618
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  • RIV/60076658:_____/02:00004048
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  • Gliocladium spp. Gliocladium virens; Paecilomyces fumosoroseus; Verticillium lecanii; entomopathogenic fungi; mycoparasitic fungi; identification using RAPD-PCR (en)
http://linked.open.../riv/klicoveSlovo
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  • CZ - Česká republika
http://linked.open...ontrolniKodProRIV
  • [A0132F943335]
http://linked.open...i/riv/nazevZdroje
  • Plant Protection Science
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
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http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 38
http://linked.open...iv/tvurceVysledku
  • Čurn, Vladislav
  • Landa, Zdeněk
  • Osborne, L. S.
  • Bielíková, Lenka
http://linked.open...n/vavai/riv/zamer
issn
  • 1212-2580
number of pages
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