| Attributes | Values |
|---|
| rdf:type
| |
| Description
| - Práce obsahuje metody pro ex situ ochranu genových zdrojů devíti linií českého kapra obecného. Vyvinuty byly metody zmražování zachovávající vysokou motilitu po rozmražení stejně jako vysokou hodnotu velocity, fertilizace a úspěšnost líhnutí. (cs)
- In this study, cryopreservation methods were elaborated for ex situ conservation of nine strains of Bohemian common carp. A sperm freezing procedure was developed that resulted in high motility after thawing as well as high values of velocity and percentage of sperm motility, fertilization and hatching yield.Common carp sperm were diluted in Kurokura medium, chilled to 4 oC and dimethylsulfoxide (Me2SO) was added. Cryotubes of sperm with media were then cooled from +4 oC to -9 oC at a rate of 4 oC.min-1, then from -9 oC to -80 oC at a rate of 11 oC.min-1, held for 6 min at -80 oC and finally transferred into liquid N2. The spermatozoa were thawed in a water bath at 35 oC for 110 s and checked for fertilization yield, hatching yield of embryos and larval malformations. Fresh and frozen/thawed sperm were evaluated for the percentage and for the velocity of motile sperm from video frames using image analysis (Linhart et al., 2000).The means with standard deviation (SD) were evaluated from triplicates. A
- In this study, cryopreservation methods were elaborated for ex situ conservation of nine strains of Bohemian common carp. A sperm freezing procedure was developed that resulted in high motility after thawing as well as high values of velocity and percentage of sperm motility, fertilization and hatching yield.Common carp sperm were diluted in Kurokura medium, chilled to 4 oC and dimethylsulfoxide (Me2SO) was added. Cryotubes of sperm with media were then cooled from +4 oC to -9 oC at a rate of 4 oC.min-1, then from -9 oC to -80 oC at a rate of 11 oC.min-1, held for 6 min at -80 oC and finally transferred into liquid N2. The spermatozoa were thawed in a water bath at 35 oC for 110 s and checked for fertilization yield, hatching yield of embryos and larval malformations. Fresh and frozen/thawed sperm were evaluated for the percentage and for the velocity of motile sperm from video frames using image analysis (Linhart et al., 2000).The means with standard deviation (SD) were evaluated from triplicates. A (en)
|
| Title
| - Cryokonzervace spematu kapra obecného (Cyprinus carpio L.): vztah mezi motilitou spermií a líhnivosti plůdku (cs)
- Cryopreservation of sperm in common carp Cyprinus carpio: correlation of sperm motility and hatching of embryos
- Cryopreservation of sperm in common carp Cyprinus carpio: correlation of sperm motility and hatching of embryos (en)
|
| skos:prefLabel
| - Cryokonzervace spematu kapra obecného (Cyprinus carpio L.): vztah mezi motilitou spermií a líhnivosti plůdku (cs)
- Cryopreservation of sperm in common carp Cyprinus carpio: correlation of sperm motility and hatching of embryos
- Cryopreservation of sperm in common carp Cyprinus carpio: correlation of sperm motility and hatching of embryos (en)
|
| skos:notation
| - RIV/60076658:12610/02:00005782!RIV/2005/GA0/126105/N
|
| http://linked.open.../vavai/riv/strany
| |
| http://linked.open...avai/riv/aktivita
| |
| http://linked.open...avai/riv/aktivity
| - P(GV523/97/S056), Z(MSM 126100001)
|
| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
| |
| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
| |
| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/60076658:12610/02:00005782
|
| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - cryopreservation;sperm;common carp;Cyprinus carpio;motility;hatching (en)
|
| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...v/mistoKonaniAkce
| |
| http://linked.open...i/riv/mistoVydani
| |
| http://linked.open...i/riv/nazevZdroje
| - XX -th Genetic Days - Proceedings of the international scientifis conference
|
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...iv/tvurceVysledku
| - Linhart, Otomar
- Rodina, Marek
|
| http://linked.open...vavai/riv/typAkce
| |
| http://linked.open.../riv/zahajeniAkce
| |
| http://linked.open...n/vavai/riv/zamer
| |
| number of pages
| |
| http://purl.org/ne...btex#hasPublisher
| - Mendelova zemědělská a lesnická univerzita v Brně
|
| https://schema.org/isbn
| |
| http://localhost/t...ganizacniJednotka
| |
![[RDF Data]](/fct/images/sw-rdf-blue.png)
![[cxml]](/fct/images/cxml_doc.png)
![[csv]](/fct/images/csv_doc.png)
![[text]](/fct/images/ntriples_doc.png)
![[turtle]](/fct/images/n3turtle_doc.png)
![[ld+json]](/fct/images/jsonld_doc.png)
![[rdf+json]](/fct/images/json_doc.png)
![[rdf+xml]](/fct/images/xml_doc.png)
![[atom+xml]](/fct/images/atom_doc.png)
![[html]](/fct/images/html_doc.png)