About: Verapamil does not modify catalytic activity of CYP450 in rainbow trout after long-term exposure

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About: Verapamil does not modify catalytic activity of CYP450 in rainbow trout after long-term exposure Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Little is known about the effects of the cardiovascular drug verapamil (VRP) on metabolic processes in fish. Most calcium channel blockers including VRP are metabolized by cytochrome P450 (CYP450) enzymes. In this study we investigated the in vivo effect of VRP on some CYP450-mediated reactions in juvenile rainbow trout (Oncorhynchus mykiss). Fish were exposed to sublethal concentrations of VRP (0.5, 27 and 270 mu g l(-1)) for 0, 21, and 42 day. The following CYP450-mediated reactions were studied in hepatic microsomes: O-dealkylation of ethoxyresorufin, methoxyresorufin, and pentoxyresorufin, hydroxylation of coumarin, tolbutamide, and p-nitrophenol, and O-debenzylation of 7-benzyloxy-4-trifluoromethylcoumarin. The amounts of products of these reactions did not differ among fish exposed to different levels of VRP and control fish. This suggests that the levels of VPR used did not alter catalytic activity of the selected CYP450 enzymes. In conclusion, none of the investigated CYP450-mediated reactions has potential as a biomarker to monitor VRP contamination of the aquatic environment. Little is known about the effects of the cardiovascular drug verapamil (VRP) on metabolic processes in fish. Most calcium channel blockers including VRP are metabolized by cytochrome P450 (CYP450) enzymes. In this study we investigated the in vivo effect of VRP on some CYP450-mediated reactions in juvenile rainbow trout (Oncorhynchus mykiss). Fish were exposed to sublethal concentrations of VRP (0.5, 27 and 270 mu g l(-1)) for 0, 21, and 42 day. The following CYP450-mediated reactions were studied in hepatic microsomes: O-dealkylation of ethoxyresorufin, methoxyresorufin, and pentoxyresorufin, hydroxylation of coumarin, tolbutamide, and p-nitrophenol, and O-debenzylation of 7-benzyloxy-4-trifluoromethylcoumarin. The amounts of products of these reactions did not differ among fish exposed to different levels of VRP and control fish. This suggests that the levels of VPR used did not alter catalytic activity of the selected CYP450 enzymes. In conclusion, none of the investigated CYP450-mediated reactions has potential as a biomarker to monitor VRP contamination of the aquatic environment. (en)
Title	Verapamil does not modify catalytic activity of CYP450 in rainbow trout after long-term exposure Verapamil does not modify catalytic activity of CYP450 in rainbow trout after long-term exposure (en)
skos:prefLabel	Verapamil does not modify catalytic activity of CYP450 in rainbow trout after long-term exposure Verapamil does not modify catalytic activity of CYP450 in rainbow trout after long-term exposure (en)
skos:notation	RIV/60076658:12520/12:43883467!RIV13-GA0-12520___
http://linked.open...avai/predkladatel	Fakulta rybářství a ochrany vod
http://linked.open...avai/riv/aktivita	P S
http://linked.open...avai/riv/aktivity	P(ED2.1.00/01.0024), P(GAP503/11/1130), S
http://linked.open...iv/cisloPeriodika	05
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Burkina, Viktoriia Randák, Tomáš Žlábek, Vladimír Fedorova, Ganna Li, Zhihua
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Jihočeská univerzita v Českých Budějovicích / Fakulta rybářství a ochrany vod
http://linked.open...dnocenehoVysledku	177227
http://linked.open...ai/riv/idVysledku	RIV/60076658:12520/12:43883467
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Biomarker; Rainbow trout; Hepatic microsomes; Cytochrome P450; Verapamil (en)
http://linked.open.../riv/klicoveSlovo	Biomarker Hepatic microsomes Cytochrome P450 Rainbow trout Verapamil
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[A1C802159CA0]
http://linked.open...i/riv/nazevZdroje	ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
http://linked.open...in/vavai/riv/obor	GL
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Effect of environmental levels of selected pharmaceuticals on rainbow trout (Oncorhynchus mykiss) and fish cell culture models South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	79
http://linked.open...iv/tvurceVysledku	Fedorova, Ganna Li, Zhihua Pickova, J. Randák, Tomáš Žlábek, Vladimír Zamaratskaia, G. Burkina, Viktoriia
http://linked.open...ain/vavai/riv/wos	000302107000021
issn	0147-6513
number of pages	5 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.ecoenv.2011.12.012
http://localhost/t...ganizacniJednotka	12520
is http://linked.open...avai/riv/vysledek of	Verapamil does not modify catalytic activity of CYP450 in rainbow trout after long-term exposure

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