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| rdf:type
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| Description
| - Distinct crystalloids were found in chloroplasts of transgenic Pssu-ipt tobacco (Nicotiana tabacum L. cv. Petit Havana SR1) overproducing endogenous cytokinins. They were present both in rooted (T) and grafted (TC) transgenic plants contrary to control tobacco (C). The fractions enriched by crystalloids were isolated from chloroplasts using a continuous or a discontinuous Percoll gradient. Chlorophyll (Chl) fluorescence emission spectra at 77 K indicated the presence of aggregates of light-harvesting complex proteins (LHC2) that was not connected to reaction centres of photosystem 2 both in isolated chloroplasts and in the fraction of 80% Percoll gradient from both types of transgenic tobacco. Further analyses, i.e. pigment contents, polypeptide composition by SDS-PAGE, and immunoblotting Support our hypothesis that crystalloids inside chloroplasts of transgenic tobacco are formed by LHC2 aggregates. Treatment with two distinct detergents, chosen with respect to their effects (i.e. beta-dodecyl maltos
- Distinct crystalloids were found in chloroplasts of transgenic Pssu-ipt tobacco (Nicotiana tabacum L. cv. Petit Havana SR1) overproducing endogenous cytokinins. They were present both in rooted (T) and grafted (TC) transgenic plants contrary to control tobacco (C). The fractions enriched by crystalloids were isolated from chloroplasts using a continuous or a discontinuous Percoll gradient. Chlorophyll (Chl) fluorescence emission spectra at 77 K indicated the presence of aggregates of light-harvesting complex proteins (LHC2) that was not connected to reaction centres of photosystem 2 both in isolated chloroplasts and in the fraction of 80% Percoll gradient from both types of transgenic tobacco. Further analyses, i.e. pigment contents, polypeptide composition by SDS-PAGE, and immunoblotting Support our hypothesis that crystalloids inside chloroplasts of transgenic tobacco are formed by LHC2 aggregates. Treatment with two distinct detergents, chosen with respect to their effects (i.e. beta-dodecyl maltos (en)
- Izolovali a charakterizovali jsme pseudokrystalické struktury vyskytující se v transgenních rostlinách tabáku Pssu-ipt. Prokázali jsme, že jsou tvořeny uspořádanými agregáty světlosběrného komplexu fotosystému II. (cs)
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| Title
| - Isolation and characterization of paracrystalline structures from transgenic Pssu-ipt tobacco
- Isolation and characterization of paracrystalline structures from transgenic Pssu-ipt tobacco (en)
- Izolace a charakterizace parakrystalických struktur z transgenních tabáků Pssu-ipt (cs)
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| skos:prefLabel
| - Isolation and characterization of paracrystalline structures from transgenic Pssu-ipt tobacco
- Isolation and characterization of paracrystalline structures from transgenic Pssu-ipt tobacco (en)
- Izolace a charakterizace parakrystalických struktur z transgenních tabáků Pssu-ipt (cs)
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| skos:notation
| - RIV/60076658:12310/05:00008748!RIV08-MSM-12310___
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(GA206/01/1061), P(GA206/03/0369), P(GA206/03/1107), V, Z(AV0Z50380511)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/60076658:12310/05:00008748
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - aggregates; chloroplast ultrastructure; cytokinins; fluorescence emission spectra; light-harvesting complex proteins; Nicotiana; three-dimensional reconstruction light-harvesting complex; chlorophyll-a/b complex; thylakoid membranes; photosystem-II; intact leaves; chloroplasts; aggregation; proteins; ultrastructure; cotyledons (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Vácha, František
- Bumba, Ladislav
- Synková, H.
- Čeřovská, N.
- Hušák, Michal
- Šiffel, Pavel
- Hunalová, I.
- Schnablová, R.
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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| http://localhost/t...ganizacniJednotka
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| is http://linked.open...avai/riv/vysledek
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