About: Genetic analysis of the ADGF multigene family by homologous recombination and gene conversion in Drosophila

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About: Genetic analysis of the ADGF multigene family by homologous recombination and gene conversion in Drosophila Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Genetická analýza multigenové rodiny ADGF homologní rekombinací a konverzí genu u Drosofila (cs) Many Drosophila genes exist as members of multigene families and within each family the members can be functionally redundant, making it difficult to identify them by classical mutagenesis techniques based on phenotypic screening. We have addressed this problem in a genetic analysis of a novel family of six adenosine deaminase-related growth factors (ADGFs). We used ends-in targeting to introduce mutations into five of the six ADGF genes, taking advantage of the fact that five of the family members are encoded by a three-gene cluster and a two-gene cluster. We used two targeting constructs to introduce loss-of-function mutations into all five genes, as well as to isolate different combinations of multiple mutations, independent of phenotypic consequences. The results show that (1) it is possible to use ends-in targeting to disrupt gene clusters; (2) gene conversion, which is usually considered a complication in gene targeting, can be used to help recover different mutant combinations in a single scree Many Drosophila genes exist as members of multigene families and within each family the members can be functionally redundant, making it difficult to identify them by classical mutagenesis techniques based on phenotypic screening. We have addressed this problem in a genetic analysis of a novel family of six adenosine deaminase-related growth factors (ADGFs). We used ends-in targeting to introduce mutations into five of the six ADGF genes, taking advantage of the fact that five of the family members are encoded by a three-gene cluster and a two-gene cluster. We used two targeting constructs to introduce loss-of-function mutations into all five genes, as well as to isolate different combinations of multiple mutations, independent of phenotypic consequences. The results show that (1) it is possible to use ends-in targeting to disrupt gene clusters; (2) gene conversion, which is usually considered a complication in gene targeting, can be used to help recover different mutant combinations in a single scree (en)
Title	Genetic analysis of the ADGF multigene family by homologous recombination and gene conversion in Drosophila Genetic analysis of the ADGF multigene family by homologous recombination and gene conversion in Drosophila (en) Genetická analýza multigenové rodiny ADGF homologní rekombinací a konverzí genu u Drosofila (cs)
skos:prefLabel	Genetic analysis of the ADGF multigene family by homologous recombination and gene conversion in Drosophila Genetic analysis of the ADGF multigene family by homologous recombination and gene conversion in Drosophila (en) Genetická analýza multigenové rodiny ADGF homologní rekombinací a konverzí genu u Drosofila (cs)
skos:notation	RIV/60076658:12310/03:00008619!RIV08-MSM-12310___
http://linked.open.../vavai/riv/strany	653-666
http://linked.open...avai/riv/aktivita	P V Z
http://linked.open...avai/riv/aktivity	P(GA204/01/1022), P(IAA5007107), P(ME 549), V, Z(AV0Z5007907)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2008
http://linked.open...aciTvurceVysledku	Žurovec, Michal
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Jihočeská univerzita v Českých Budějovicích / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	608131
http://linked.open...ai/riv/idVysledku	RIV/60076658:12310/03:00008619
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	GROWTH-FACTOR; MELANOGASTER; ADENOSINE; KNOCKOUT; PATHWAYS; BREAKS; REPAIR (en)
http://linked.open.../riv/klicoveSlovo	REPAIR BREAKS GROWTH-FACTOR PATHWAYS ADENOSINE KNOCKOUT MELANOGASTER
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[F2F6AAB00236]
http://linked.open...i/riv/nazevZdroje	Genetics
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Genetic Analysis of Drosophila Growth Factors Adenosine deaminase homologs in Drosophila http://linked.opendata.cz/resource/domain/vavai/projekt/ME%20549
http://linked.open...UplatneniVysledku	2003
http://linked.open...v/svazekPeriodika	165
http://linked.open...iv/tvurceVysledku	Žurovec, Michal Doležal, Tomáš Gaži, Michal Bryant, Peter J.
http://linked.open...n/vavai/riv/zamer	The use of insects for quality assessment and possible management of the ecosystems in Central Europe
issn	0016-6731
number of pages	14 (xsd:int)
http://localhost/t...ganizacniJednotka	12310

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