About: LC/MS analysis of adipokinetic peptides in the Pyrrhocoris apterus (Heteroptera)

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About: LC/MS analysis of adipokinetic peptides in the Pyrrhocoris apterus (Heteroptera) Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	An LC/ESI/MS/MS method was developed for quantitative analysis of peptides from the adipokinetic hormone/red pigment concentrating hormone family (AKH/RPCH family) in insects. The peptides were extracted by aqueous methanol, the extract defated and the aqueous layer analysed by LC/ESI/MSN. With the developed methodology low femtomole levels can be detected allowing thus direct AKH sequencing and analysis in the corpora cardiaca from one individual insect. Direct examination of corpora cardiaca of the P. apterus revealed a new adipokinetic peptide. It is an octapeptide designated as Pya-AKH-II with the sequence of pGlu-Leu-Thr-Phe-Thr-Pro-Asn-Trp-NH2. The new peptide is active in the lipid mobilisation assay, but its activity seems to be lower than with the Pya-AKH-I. The identity of the Pya-AKH-II was definitively confirmed by synthesis. An LC/ESI/MS/MS method was developed for quantitative analysis of peptides from the adipokinetic hormone/red pigment concentrating hormone family (AKH/RPCH family) in insects. The peptides were extracted by aqueous methanol, the extract defated and the aqueous layer analysed by LC/ESI/MSN. With the developed methodology low femtomole levels can be detected allowing thus direct AKH sequencing and analysis in the corpora cardiaca from one individual insect. Direct examination of corpora cardiaca of the P. apterus revealed a new adipokinetic peptide. It is an octapeptide designated as Pya-AKH-II with the sequence of pGlu-Leu-Thr-Phe-Thr-Pro-Asn-Trp-NH2. The new peptide is active in the lipid mobilisation assay, but its activity seems to be lower than with the Pya-AKH-I. The identity of the Pya-AKH-II was definitively confirmed by synthesis. (en)
Title	LC/MS analysis of adipokinetic peptides in the Pyrrhocoris apterus (Heteroptera) LC/MS analysis of adipokinetic peptides in the Pyrrhocoris apterus (Heteroptera) (en)
skos:prefLabel	LC/MS analysis of adipokinetic peptides in the Pyrrhocoris apterus (Heteroptera) LC/MS analysis of adipokinetic peptides in the Pyrrhocoris apterus (Heteroptera) (en)
skos:notation	RIV/60076658:12310/01:00003161!RIV/2002/AV0/123102/N
http://linked.open.../vavai/riv/strany	90-92
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA203/01/1586), P(ME 417), Z(AV0Z5007907)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2002
http://linked.open...aciTvurceVysledku	Kodrík, Dalibor
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Jihočeská univerzita v Českých Budějovicích / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	685123
http://linked.open...ai/riv/idVysledku	RIV/60076658:12310/01:00003161
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	insect peptides; AKH/RPC family; P. apterus; AKH quantitative analysis (en)
http://linked.open.../riv/klicoveSlovo	AKH quantitative analysis AKH/RPC family P. apterus insect peptides
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[330992EB6E72]
http://linked.open...i/riv/nazevZdroje	Collection Symposium Series
http://linked.open...in/vavai/riv/obor	ED
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	Alkyl chloroformate-induced derivatization in sample preparation and analysis of compounds of biomedical and biological importance http://linked.opendata.cz/resource/domain/vavai/projekt/ME%20417
http://linked.open...UplatneniVysledku	2001
http://linked.open...v/svazekPeriodika	4
http://linked.open...iv/tvurceVysledku	Kodrík, Dalibor Šimek, P. Lepša, L.
http://linked.open...n/vavai/riv/zamer	The use of insects for quality assessment and possible management of the ecosystems in Central Europe
number of pages	3 (xsd:int)
http://localhost/t...ganizacniJednotka	12310

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