About: Selection of indigenous isolates of entomopathogenic soil fungus Metarhizium anisopliae under laboratory conditions     Goto   Sponge   NotDistinct   Permalink

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  • Eight native isolates of the entomopathogenic fungus Metarhizium anisopliae (Metschnikoff) Sorokin were obtained by monitoring soils cultivated in a conventional manner. These isolates were compared in three areas: (a) conidial germination, (b) radial growth and sporulation and (c) ability of conidia to infect Tenebrio molitor larvae. All bioassays were carried out at constant temperatures of 10, 15, and 20 A degrees C. Conidia of individual isolates demonstrated differences in germination after a 24-h long incubation at all evaluated temperatures. At 20 A degrees C, the germination ranged from 67 to 100 % and at 15 A degrees C from 5.33 to 46.67 %. At 10 A degrees C, no germination was observed after 24 h; nevertheless, it was 8.67-44.67 % after 48 h. In terms of radial growth, the culture diameters and the associated production of spores of all isolates increased with increasing temperature. At 10 A degrees C, sporulation was observed in three isolates while all remaining cultures appeared sterile. Three weeks post-inoculation, conidia of all assessed isolates caused 100 % cumulative mortality of treated larvae of T. molitor at 15 and 20 A degrees C with the exception of isolate 110108 that induced 81.33 % mortality at 15 A degrees C. At 10 A degrees C, larval cumulative mortality ranged from 6.67 to 85.33 % depending on the isolate. Isolates 110108 and 110111 showed significantly slower outset and a much lower rate of infection at all temperatures compared to other tested isolates of M. anisopliae. The bioassays were carried out with the purpose to sort and select indigenous isolates of M. anisopliae useful as biocontrol agents in their original habitat.
  • Eight native isolates of the entomopathogenic fungus Metarhizium anisopliae (Metschnikoff) Sorokin were obtained by monitoring soils cultivated in a conventional manner. These isolates were compared in three areas: (a) conidial germination, (b) radial growth and sporulation and (c) ability of conidia to infect Tenebrio molitor larvae. All bioassays were carried out at constant temperatures of 10, 15, and 20 A degrees C. Conidia of individual isolates demonstrated differences in germination after a 24-h long incubation at all evaluated temperatures. At 20 A degrees C, the germination ranged from 67 to 100 % and at 15 A degrees C from 5.33 to 46.67 %. At 10 A degrees C, no germination was observed after 24 h; nevertheless, it was 8.67-44.67 % after 48 h. In terms of radial growth, the culture diameters and the associated production of spores of all isolates increased with increasing temperature. At 10 A degrees C, sporulation was observed in three isolates while all remaining cultures appeared sterile. Three weeks post-inoculation, conidia of all assessed isolates caused 100 % cumulative mortality of treated larvae of T. molitor at 15 and 20 A degrees C with the exception of isolate 110108 that induced 81.33 % mortality at 15 A degrees C. At 10 A degrees C, larval cumulative mortality ranged from 6.67 to 85.33 % depending on the isolate. Isolates 110108 and 110111 showed significantly slower outset and a much lower rate of infection at all temperatures compared to other tested isolates of M. anisopliae. The bioassays were carried out with the purpose to sort and select indigenous isolates of M. anisopliae useful as biocontrol agents in their original habitat. (en)
Title
  • Selection of indigenous isolates of entomopathogenic soil fungus Metarhizium anisopliae under laboratory conditions
  • Selection of indigenous isolates of entomopathogenic soil fungus Metarhizium anisopliae under laboratory conditions (en)
skos:prefLabel
  • Selection of indigenous isolates of entomopathogenic soil fungus Metarhizium anisopliae under laboratory conditions
  • Selection of indigenous isolates of entomopathogenic soil fungus Metarhizium anisopliae under laboratory conditions (en)
skos:notation
  • RIV/60076658:12220/14:43887697!RIV15-MSM-12220___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • S, Z(MSM6007665806)
http://linked.open...iv/cisloPeriodika
  • 4
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 44286
http://linked.open...ai/riv/idVysledku
  • RIV/60076658:12220/14:43887697
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • germination; sporulation; thermotolerance; rhizosphere; temperatures; virulence; radial growth; growing media; Beauveria bassiana; Conservation biological-control (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [988BD9187173]
http://linked.open...i/riv/nazevZdroje
  • Folia Microbiologica
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 59
http://linked.open...iv/tvurceVysledku
  • Šimková, Jana
  • Bohatá, Andrea
  • Landa, Zdeněk
  • Skalický, Aleš
  • Osborne, L. S.
http://linked.open...ain/vavai/riv/wos
  • 000337031400001
http://linked.open...n/vavai/riv/zamer
issn
  • 0015-5632
number of pages
http://bibframe.org/vocab/doi
  • 10.1007/s12223-013-0293-z
http://localhost/t...ganizacniJednotka
  • 12220
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