About: Validation of microarray analysis and identification of differentially expressed genes in two durum wheat cultivars with contrasting response to drought stress

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The durum wheat cultivars ?Cappelli? and ?Ofanto? are characterized by a clear differences in response to heat and drought stress. Wheat Affymetrix microarray was used in the two cultivars subjected to water stress at booting stage. Differential expressed genes with different stress expression profiles were found in Cappelli vs. Ofanto. According to the fold change threshold of the Affymetrix dataset and the annotated activity among these genes drought response gene markers were selected and some of them have been validated by qRT-PCR for their stabile behaving during plant development in an experiment made with two cultivars under drought stress at tillering developmental stages. These results will open the possibility to identify expression QTLs controlling the response of durum wheat to abiotic stress making possible the understanding of the genetic bases of stress dependent gene expression. The durum wheat cultivars ?Cappelli? and ?Ofanto? are characterized by a clear differences in response to heat and drought stress. Wheat Affymetrix microarray was used in the two cultivars subjected to water stress at booting stage. Differential expressed genes with different stress expression profiles were found in Cappelli vs. Ofanto. According to the fold change threshold of the Affymetrix dataset and the annotated activity among these genes drought response gene markers were selected and some of them have been validated by qRT-PCR for their stabile behaving during plant development in an experiment made with two cultivars under drought stress at tillering developmental stages. These results will open the possibility to identify expression QTLs controlling the response of durum wheat to abiotic stress making possible the understanding of the genetic bases of stress dependent gene expression. (en)
Title	Validation of microarray analysis and identification of differentially expressed genes in two durum wheat cultivars with contrasting response to drought stress Validation of microarray analysis and identification of differentially expressed genes in two durum wheat cultivars with contrasting response to drought stress (en)
skos:prefLabel	Validation of microarray analysis and identification of differentially expressed genes in two durum wheat cultivars with contrasting response to drought stress Validation of microarray analysis and identification of differentially expressed genes in two durum wheat cultivars with contrasting response to drought stress (en)
skos:notation	RIV/60076658:12220/10:00012493!RIV11-GA0-12220___
http://linked.open...avai/riv/aktivita	P S
http://linked.open...avai/riv/aktivity	P(GD521/08/H042), S
http://linked.open...iv/cisloPeriodika	12
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Čurn, Vladislav Havlíčková, Lenka
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Jihočeská univerzita v Českých Budějovicích / Zemědělská fakulta
http://linked.open...dnocenehoVysledku	295196
http://linked.open...ai/riv/idVysledku	RIV/60076658:12220/10:00012493
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Triticum durum; drought stress; expression analysis; RSWC; Microarrays (en)
http://linked.open.../riv/klicoveSlovo	drought stress Triticum durum expression analysis Microarrays RSWC
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[E4D411B02D14]
http://linked.open...i/riv/nazevZdroje	Úroda : časopis pro rostlinnou produkci
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...vavai/riv/projekt	Molecular biology, ecology and taxonomy
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	58
http://linked.open...iv/tvurceVysledku	Havlíčková, Lenka Čurn, Vladislav
issn	0139-6013
number of pages	4 (xsd:int)
http://localhost/t...ganizacniJednotka	12220
is http://linked.open...avai/riv/vysledek of	Validation of microarray analysis and identification of differentially expressed genes in two durum wheat cultivars with contrasting response to drought stress

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