About: Chromatographic methods for determination of S-substituted cysteine derivatives

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	A novel HPLC method for determination of a wide variety of S-substituted cysteine derivatives in Allium species has been developed and validated. This method allows simultaneous separation and quantification of S-alk(en)ylcysteine S-oxides, γ-glutamyl-S-alk(en)ylcysteines and γ-glutamyl-S-alk(en)ylcysteine S-oxides in a single run. The procedure is based on extraction of these amino acids and dipeptides by methanol, their derivatization by dansyl chloride and subsequent separation by reversed phase HPLC. The main advantages of the new method are simplicity, excellent stability of derivatives, high sensitivity, specificity and the ability to simultaneously analyze the whole range of S-substituted cysteine derivatives. This method was critically compared with other chromatographic procedures used for quantification of S-substituted cysteine derivatives, namely with two other HPLC methods (derivatization by o-phthaldialdehyde/tert-butylthiol and fluorenylmethyl chloroformate), and with determin A novel HPLC method for determination of a wide variety of S-substituted cysteine derivatives in Allium species has been developed and validated. This method allows simultaneous separation and quantification of S-alk(en)ylcysteine S-oxides, γ-glutamyl-S-alk(en)ylcysteines and γ-glutamyl-S-alk(en)ylcysteine S-oxides in a single run. The procedure is based on extraction of these amino acids and dipeptides by methanol, their derivatization by dansyl chloride and subsequent separation by reversed phase HPLC. The main advantages of the new method are simplicity, excellent stability of derivatives, high sensitivity, specificity and the ability to simultaneously analyze the whole range of S-substituted cysteine derivatives. This method was critically compared with other chromatographic procedures used for quantification of S-substituted cysteine derivatives, namely with two other HPLC methods (derivatization by o-phthaldialdehyde/tert-butylthiol and fluorenylmethyl chloroformate), and with determin (en)
Title	Chromatographic methods for determination of S-substituted cysteine derivatives - a comparative study Chromatographic methods for determination of S-substituted cysteine derivatives - a comparative study (en)
skos:prefLabel	Chromatographic methods for determination of S-substituted cysteine derivatives - a comparative study Chromatographic methods for determination of S-substituted cysteine derivatives - a comparative study (en)
skos:notation	RIV/60076658:12220/09:00010895!RIV10-MSM-12220___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(OC 123), Z(MSM6007665806)
http://linked.open...iv/cisloPeriodika	41
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Kubec, Roman Dadáková, Eva
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Jihočeská univerzita v Českých Budějovicích / Zemědělská fakulta
http://linked.open...dnocenehoVysledku	307042
http://linked.open...ai/riv/idVysledku	RIV/60076658:12220/09:00010895
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Allium; garlic; onion; FMOC; OPA; dansyl; MEKC; alliin; isoalliin; methiin; glutamyl dipeptide. (en)
http://linked.open.../riv/klicoveSlovo	OPA MEKC onion Allium alliin garlic isoalliin methiin FMOC dansyl glutamyl dipeptide.
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[175F55E20F02]
http://linked.open...i/riv/nazevZdroje	Journal of Chromatography A
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...vavai/riv/projekt	http://linked.opendata.cz/resource/domain/vavai/projekt/OC%20123
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	1216
http://linked.open...iv/tvurceVysledku	Dadáková, Eva Kubec, Roman
http://linked.open...n/vavai/riv/zamer	Sustainable methods of agricultural management in montane and submontane areas with respect to harmonization of the production and nonproduction spheres
issn	0021-9673
number of pages	8 (xsd:int)
http://localhost/t...ganizacniJednotka	12220
is http://linked.open...avai/riv/vysledek of	Chromatographic methods for determination of S-substituted cysteine derivatives - a comparative study Chromatographic methods for determination of S-substituted cysteine derivatives - a comparative study Chromatographic methods for determination of S-substituted cysteine derivatives - a comparative study Chromatographic methods for determination of S-substituted cysteine derivatives - a comparative study

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