About: Potato Variety Identification by Molecular Markers Based on Retrotransposon Analyses

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	We analyzed a set of twenty most grown potato (Solanum tuberosum L.) varieties listed in the Czech Variety List using the PCR-IRAP (Inter-Retrotransposon Amplified Polymorphism) method in order to distinguish fast and unambiguously the varieties. In total, 62 polymorphic alleles were amplified using the three primers P-Tst-1, P-Tst-3 and P-Tst-6. The recorded pattern of markers was stable and reproducible. The analyses were repeated three times and identical results were always obtained. The level of polymorphism varied from 11% to 79% depending on the respective primer. All analysed varieties could be reliably distinguished after multivariate statistics have been applied to the data obtained by the PCO and UPGMA analyses. The best resolution of individual varieties was obtained if all three primers were evaluated as a complex. The use of retrotransposon-based markers appears to be suitable for the differentiation of large sets of potato samples and should be an eligible complement to other molecular We analyzed a set of twenty most grown potato (Solanum tuberosum L.) varieties listed in the Czech Variety List using the PCR-IRAP (Inter-Retrotransposon Amplified Polymorphism) method in order to distinguish fast and unambiguously the varieties. In total, 62 polymorphic alleles were amplified using the three primers P-Tst-1, P-Tst-3 and P-Tst-6. The recorded pattern of markers was stable and reproducible. The analyses were repeated three times and identical results were always obtained. The level of polymorphism varied from 11% to 79% depending on the respective primer. All analysed varieties could be reliably distinguished after multivariate statistics have been applied to the data obtained by the PCO and UPGMA analyses. The best resolution of individual varieties was obtained if all three primers were evaluated as a complex. The use of retrotransposon-based markers appears to be suitable for the differentiation of large sets of potato samples and should be an eligible complement to other molecular (en)
Title	Potato Variety Identification by Molecular Markers Based on Retrotransposon Analyses Potato Variety Identification by Molecular Markers Based on Retrotransposon Analyses (en)
skos:prefLabel	Potato Variety Identification by Molecular Markers Based on Retrotransposon Analyses Potato Variety Identification by Molecular Markers Based on Retrotransposon Analyses (en)
skos:notation	RIV/60076658:12220/09:00010856!RIV10-MZE-12220___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(1B44011), P(GD521/08/H042), Z(MSM6007665806)
http://linked.open...iv/cisloPeriodika	45
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Bárta, Jan Čurn, Vladislav Šimáčková, Kateřina Nováková, Alena
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Jihočeská univerzita v Českých Budějovicích / Zemědělská fakulta
http://linked.open...dnocenehoVysledku	334786
http://linked.open...ai/riv/idVysledku	RIV/60076658:12220/09:00010856
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	molecular markers; AFLP; SSR (en)
http://linked.open.../riv/klicoveSlovo	SSR molecular markers AFLP
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[4D5B9CC2856C]
http://linked.open...i/riv/nazevZdroje	Czech Journal of Genetics and Plant Breeding : genetika a šlechtění
http://linked.open...in/vavai/riv/obor	EI
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Molecular biology, ecology and taxonomy Development and testing of the system of analytical methods for practical characterization of potato cultivars registered in the Czech Republic
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	2009
http://linked.open...iv/tvurceVysledku	Bárta, Jan Nováková, Alena Šimáčková, Kateřina Čurn, Vladislav
http://linked.open...n/vavai/riv/zamer	Sustainable methods of agricultural management in montane and submontane areas with respect to harmonization of the production and nonproduction spheres
issn	1212-1975
number of pages	11 (xsd:int)
http://localhost/t...ganizacniJednotka	12220
is http://linked.open...avai/riv/vysledek of	Potato Variety Identification by Molecular Markers Based on Retrotransposon Analyses Potato Variety Identification by Molecular Markers Based on Retrotransposon Analyses Potato Variety Identification by Molecular Markers Based on Retrotransposon Analyses Potato Variety Identification by Molecular Markers Based on Retrotransposon Analyses

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