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| Description
| - The study was aimed at examining the effects of IGF-I on ram sperm traits after hypothermic (4ºC) storage. Sperm ejaculates from 3 Lacaune rams were diluted in Triladyl, pooled and divided into the groups of IGF-I doses added (0, 10, 100 or 200 ng/ml). Following 72 h of storage the sperm samples were stained for a plasma membrane integrity (peanut agglutinin, PNA-FITC), membrane stability (annexin V-FITC) and apoptosis (Yo-Pro-1) and analyzed under a fluorescent microscope. Sperm motility was determined by CASA (at 0, 24 and 48h) and fertilizing ability (at 48h) - by an in vitro fertilization (IVF) test on bovine oocytes. IGF-I (100 and 200 ng/ml) decreased the portion of spermatozoa with disrupted plasma membrane integrity (15.9 and 16.5% resp. vs 28.3% in control, p< 0.05) and, given at all tested doses, reduced the rate of apoptotic sperm (6.2, 4.9 and 4.4% resp. vs 9.7% in control, p< 0.05). The proportion of spermatozoa with annexin V-labelled membrane changes was reduced by IGF-I from 9.6% (control) to 4.9% (100 ng/ml) and 6.1% (200 ng/ml). IGF-I (10 ng/ml), elevated sperm motility measured after 24 h storage (83.3% vs. 69.2% in control); the higher doses had no effect. IGF-I, given at 100 ng/ml, increased sperm fertilization rate (67.8% vs. 47.0%, p<0.05). In summary, IGF-I improved ram sperm functions during cooling storage and their effects were reflected in fertilizing ability in vitro.
- The study was aimed at examining the effects of IGF-I on ram sperm traits after hypothermic (4ºC) storage. Sperm ejaculates from 3 Lacaune rams were diluted in Triladyl, pooled and divided into the groups of IGF-I doses added (0, 10, 100 or 200 ng/ml). Following 72 h of storage the sperm samples were stained for a plasma membrane integrity (peanut agglutinin, PNA-FITC), membrane stability (annexin V-FITC) and apoptosis (Yo-Pro-1) and analyzed under a fluorescent microscope. Sperm motility was determined by CASA (at 0, 24 and 48h) and fertilizing ability (at 48h) - by an in vitro fertilization (IVF) test on bovine oocytes. IGF-I (100 and 200 ng/ml) decreased the portion of spermatozoa with disrupted plasma membrane integrity (15.9 and 16.5% resp. vs 28.3% in control, p< 0.05) and, given at all tested doses, reduced the rate of apoptotic sperm (6.2, 4.9 and 4.4% resp. vs 9.7% in control, p< 0.05). The proportion of spermatozoa with annexin V-labelled membrane changes was reduced by IGF-I from 9.6% (control) to 4.9% (100 ng/ml) and 6.1% (200 ng/ml). IGF-I (10 ng/ml), elevated sperm motility measured after 24 h storage (83.3% vs. 69.2% in control); the higher doses had no effect. IGF-I, given at 100 ng/ml, increased sperm fertilization rate (67.8% vs. 47.0%, p<0.05). In summary, IGF-I improved ram sperm functions during cooling storage and their effects were reflected in fertilizing ability in vitro. (en)
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| Title
| - Effect of insulin-like growth factor I on functional parameters of ram cooled-stored spermatozoa
- Effect of insulin-like growth factor I on functional parameters of ram cooled-stored spermatozoa (en)
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| skos:prefLabel
| - Effect of insulin-like growth factor I on functional parameters of ram cooled-stored spermatozoa
- Effect of insulin-like growth factor I on functional parameters of ram cooled-stored spermatozoa (en)
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| skos:notation
| - RIV/49608851:_____/12:#0000581!RIV13-MZE-49608851
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| http://linked.open...avai/predkladatel
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/49608851:_____/12:#0000581
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - ram; sperm; IGF-I; viability; motility; fertilizing ability (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
| - GB - Spojené království Velké Británie a Severního Irska
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...iv/tvurceVysledku
| - Hegedüšová, Zdeňka
- Kubovičová, Elena
- Makarevič, Alexander
- Špaleková, Eliška
- Olexiková, Lucia
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| issn
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| number of pages
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| http://bibframe.org/vocab/doi
| - 10.1017/S0967199412000500
|
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