About: Rep-PCR-based typing as a tool for tracking of MRSA infection origin

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	A performance of rep-PCR fi ngerprinting method with the (GTG)5 primer was explored in order to evaluate its practical application for confi rmation the source of human staphylococci infection. A laboratory worker daily working with the MRSA and Staphylococcus aureus strains has been tested positively for MRSA nosocomial infection. The collection of nine MRSA strains held in the laboratory has been typed with the rep-PCR method. Analysis of the fi ngerprints with the unweighted pairgroup method using arithmetic averages (UPGMA) clustering method revealed presence of six strain clusters with similarity lower than 85%. The fi ngerprint of MRSA strain infecting the human diff ered signifi cantly from remaining fi ngerprints. This provided clear evidence that MRSA strain infecting the personal did not come from the laboratory strains collection. Our experimental results showed rep-PCR with the (GTG)5 primer as an eff ective tool applicable for diff erentiation of individual S. aureus strains. However, the reproducibility and discrimination power of the method depended on strict observance of the optimal PCR time and temperature profi le and PCR composition as well. A performance of rep-PCR fi ngerprinting method with the (GTG)5 primer was explored in order to evaluate its practical application for confi rmation the source of human staphylococci infection. A laboratory worker daily working with the MRSA and Staphylococcus aureus strains has been tested positively for MRSA nosocomial infection. The collection of nine MRSA strains held in the laboratory has been typed with the rep-PCR method. Analysis of the fi ngerprints with the unweighted pairgroup method using arithmetic averages (UPGMA) clustering method revealed presence of six strain clusters with similarity lower than 85%. The fi ngerprint of MRSA strain infecting the human diff ered signifi cantly from remaining fi ngerprints. This provided clear evidence that MRSA strain infecting the personal did not come from the laboratory strains collection. Our experimental results showed rep-PCR with the (GTG)5 primer as an eff ective tool applicable for diff erentiation of individual S. aureus strains. However, the reproducibility and discrimination power of the method depended on strict observance of the optimal PCR time and temperature profi le and PCR composition as well. (en)
Title	Rep-PCR-based typing as a tool for tracking of MRSA infection origin Rep-PCR-based typing as a tool for tracking of MRSA infection origin (en)
skos:prefLabel	Rep-PCR-based typing as a tool for tracking of MRSA infection origin Rep-PCR-based typing as a tool for tracking of MRSA infection origin (en)
skos:notation	RIV/49608851:_____/12:#0000559!RIV13-MZE-49608851
http://linked.open...avai/predkladatel	Výzkumný ústav pro chov skotu, s.r.o.
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(LA10029), P(QH81111)
http://linked.open...iv/cisloPeriodika	6
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Manga, Ivan Vyletělová, Marcela
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav pro chov skotu, s.r.o.
http://linked.open...dnocenehoVysledku	165055
http://linked.open...ai/riv/idVysledku	RIV/49608851:_____/12:#0000559
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	rep-PCR; fingerprinting; MRSA; infection (en)
http://linked.open.../riv/klicoveSlovo	MRSA infection fingerprinting rep-PCR
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[99D271F660DE]
http://linked.open...i/riv/nazevZdroje	Acta Universitatis Agriculturae et Silviculturae Mendeleianae Brunensis
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...vavai/riv/projekt	Monitoring of prevalence and ethiology of methicillin-resistant staphylococci, and ways of their dissemination within the dairy cows, goats and sheep. Support of the membership and cooperation with the Society for Applied Microbiology (SfAM).
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	60
http://linked.open...iv/tvurceVysledku	Manga, Ivan Vyletělová, Marcela
issn	1211-8516
number of pages	6 (xsd:int)

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