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Description
| - Cílem práce bylo prověřit zda vystavení bovinních embryí vlivu viru BHV-1 ovlivní jejich další vývoj a vyvolá změny v ultrastruktuře buněčných organel; jestli zóna pelucida je bariérou, která může ochránit embryo před infekcí a zda promytí embrya v trypsinu je ochranou před penetrací viru do embryí a jejich poškození.Na základě nálezů je možno konstatovat, že infekce virem BHV-1 umožňuje preimplantační vývoj bovinních embryí in vitro a proto zóna pelucida není dostatečnou bariérou proti virovým poškozením, jestliže embrya nejsou ošetřena promytím v trypsinu. (cs)
- The aim of the our study was to examine whether: the exposure of bovine embryos to the BHV-1 virus in vitro can compromise their further developmental and alter the ultrastructural morphology of cellular organelles; whether the zona pellucida /(ZP) can be a barrier protecting embryos against infection; and whether washing with typsin after exposure can prevent virus penetration inside the embryo and subsequent virus-induced damages. Taken together these findings may be suggested that BHV-1 infection compromises preimplantation development of bovine embryos in vitro and therefore the ZP may not be enough on its own to prevent virus-induced damage, unless it is not accompanied with trypsin washing.
- The aim of the our study was to examine whether: the exposure of bovine embryos to the BHV-1 virus in vitro can compromise their further developmental and alter the ultrastructural morphology of cellular organelles; whether the zona pellucida /(ZP) can be a barrier protecting embryos against infection; and whether washing with typsin after exposure can prevent virus penetration inside the embryo and subsequent virus-induced damages. Taken together these findings may be suggested that BHV-1 infection compromises preimplantation development of bovine embryos in vitro and therefore the ZP may not be enough on its own to prevent virus-induced damage, unless it is not accompanied with trypsin washing. (en)
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Title
| - Vývoj a viabilita bovinních preimplantačních embryí po infekci bovinním herpesvirem-1:immunocytochemické a ultramorfologické studie. (cs)
- Development and viability of bovine preimplantation embryos after the in vitro infection with bovine herpesvirus-1:immunocytochemical and ultrastructural studies.
- Development and viability of bovine preimplantation embryos after the in vitro infection with bovine herpesvirus-1:immunocytochemical and ultrastructural studies. (en)
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skos:prefLabel
| - Vývoj a viabilita bovinních preimplantačních embryí po infekci bovinním herpesvirem-1:immunocytochemické a ultramorfologické studie. (cs)
- Development and viability of bovine preimplantation embryos after the in vitro infection with bovine herpesvirus-1:immunocytochemical and ultrastructural studies.
- Development and viability of bovine preimplantation embryos after the in vitro infection with bovine herpesvirus-1:immunocytochemical and ultrastructural studies. (en)
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skos:notation
| - RIV/49608851:_____/07:8P009145!RIV08-MZE-49608851
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http://linked.open.../vavai/riv/strany
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/49608851:_____/07:8P009145
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - bovine herpesvirus-1; embryo; ultrastructure; trypsin washing (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
| - GB - Spojené království Velké Británie a Severního Irska
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Louda, František
- Kubovičová, Elena
- Pivko, Juraj
- Slezáková, Milena
- Chrenek, P.
- Makarevich, Alexandr
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issn
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number of pages
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