| Attributes | Values |
|---|
| rdf:type
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| Description
| - The capacity of most flax (Linum usitatissimum L.) cultivars to accumulate cadmium (Cd) brought an interest in their use in phytoextraction of soil Cd to aboveground biomass. To investigate whether overproduction of an efficient heterologous Cd-binding peptide may enhance the phytoextraction capacity of L. usitatissimum, breeding line AGT 917 was engineered to constitutively express genetic fusion of α-domain of mammalian metallothionein 1a (αMT1a) and β-glucuronidase gus gene under the control of CaMV 35S promoter. To this end, an improved transformation protocol was developed, which involved 10-min incubation of hypocotyl segments with partially removed epidermis with Agrobacterium suspension in the presence of 100 mg.l-1 acetosyringone and 200 mg.l-1 cellulase and which increased transformation efficiency 1.6-fold as compared to agroinfection without combined acetosyringone and cellulase treatment. When tested in soils amended with Cd added at concentrations of 20 and 360 mg kg-1, the mature αMT1a::gus plants accumulated more Cd than parental AGT 917: the stem Cd concentrations in the best performing αMT1/2 line were 3.3- and 1.9-fold higher, respectively. Moreover, hypocotyl explants of αMT1/2 line showed 1.7-fold higher biomass than those of AGT 917 on media containing 15 mg Cd l-1, providing that αMT1a::gus confers higher Cd tolerance to engineered plant. Thus, overproduction of metal-binding peptides appears to be viable strategy for production of L. usitatissimum with improved phytoremediation capacity.
- The capacity of most flax (Linum usitatissimum L.) cultivars to accumulate cadmium (Cd) brought an interest in their use in phytoextraction of soil Cd to aboveground biomass. To investigate whether overproduction of an efficient heterologous Cd-binding peptide may enhance the phytoextraction capacity of L. usitatissimum, breeding line AGT 917 was engineered to constitutively express genetic fusion of α-domain of mammalian metallothionein 1a (αMT1a) and β-glucuronidase gus gene under the control of CaMV 35S promoter. To this end, an improved transformation protocol was developed, which involved 10-min incubation of hypocotyl segments with partially removed epidermis with Agrobacterium suspension in the presence of 100 mg.l-1 acetosyringone and 200 mg.l-1 cellulase and which increased transformation efficiency 1.6-fold as compared to agroinfection without combined acetosyringone and cellulase treatment. When tested in soils amended with Cd added at concentrations of 20 and 360 mg kg-1, the mature αMT1a::gus plants accumulated more Cd than parental AGT 917: the stem Cd concentrations in the best performing αMT1/2 line were 3.3- and 1.9-fold higher, respectively. Moreover, hypocotyl explants of αMT1/2 line showed 1.7-fold higher biomass than those of AGT 917 on media containing 15 mg Cd l-1, providing that αMT1a::gus confers higher Cd tolerance to engineered plant. Thus, overproduction of metal-binding peptides appears to be viable strategy for production of L. usitatissimum with improved phytoremediation capacity. (en)
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| Title
| - Enhanced accumulation of cadmium in Linum usitatissimum L. plants due to overproduction of metallothionein alfa-domain as a fusion to beta-glucuronidase protein
- Enhanced accumulation of cadmium in Linum usitatissimum L. plants due to overproduction of metallothionein alfa-domain as a fusion to beta-glucuronidase protein (en)
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| skos:prefLabel
| - Enhanced accumulation of cadmium in Linum usitatissimum L. plants due to overproduction of metallothionein alfa-domain as a fusion to beta-glucuronidase protein
- Enhanced accumulation of cadmium in Linum usitatissimum L. plants due to overproduction of metallothionein alfa-domain as a fusion to beta-glucuronidase protein (en)
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| skos:notation
| - RIV/48392952:_____/13:#0000264!RIV14-MSM-48392952
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| http://linked.open...avai/predkladatel
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
| |
| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/48392952:_____/13:#0000264
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| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - Linum usitatissimum L.; cadmium; flax; linseed; metallothionein; phytoextraction (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
| - DE - Spolková republika Německo
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Plant Cell Tissue and Organ Culture
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| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Griga, Miroslav
- Horáček, Jiří
- Kotrba, Pavel
- Smýkalová, Iva
- Smýkal, Petr
- Vrbová, Miroslava
- Švábová, Lenka
- Větrovcová, Martina
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| http://linked.open...ain/vavai/riv/wos
| |
| issn
| |
| number of pages
| |
| http://bibframe.org/vocab/doi
| - 10.1007/s11240-012-0239-1
|
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