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Description
  • A set of PEMV and PSbMV isolates collected on pea plants in five locations in the Czech Republic (determined by symptoms on pea plants and confirmed by ELISA) was characterized by biological and molecular tools. The most severe isolates were selected for sequencing and the development of constructs for pea transformation. The strategy of induction resistance to viruses was based on pathogen-derived resistance (PDR) and post-transcriptional gene silencing (PTGS). Commercial cultivars of peas were transformed and T1 seed generations of GUS/PCR positive transgenic plants were obtained. Viral infection of the progeny of pea plants will be studied further.
  • A set of PEMV and PSbMV isolates collected on pea plants in five locations in the Czech Republic (determined by symptoms on pea plants and confirmed by ELISA) was characterized by biological and molecular tools. The most severe isolates were selected for sequencing and the development of constructs for pea transformation. The strategy of induction resistance to viruses was based on pathogen-derived resistance (PDR) and post-transcriptional gene silencing (PTGS). Commercial cultivars of peas were transformed and T1 seed generations of GUS/PCR positive transgenic plants were obtained. Viral infection of the progeny of pea plants will be studied further. (en)
  • Na 5 lokalitách ČR by uskutečněn sběr izolátů virových patogenů PEMV a PSbMV (determinace symptomy na rostlinách a ELISA), které byly dále charakterizovány biologickými a molekulárními testy. Izoláty s nejvyšší patogenitou bly vybrány pro sekvenování a vývoj konstruktu pro transformaci hrachu. Strategie indukce rezistence byla založena na pathogen-derived resistance (PDR) a post-transkripčním urišování genů (PTGS). Komerční odrůdy byly transformovány a byly získány T1 rostliny s GUS/PCR pozitivní reakcí. Na těchto rostlinách bude dále studována reakce na virovou infekci. (cs)
Title
  • Švábová L., Griga M., Smýkal P., Hanáček P., Reinöhl V., Navrátil M., Šafářová D., Piáková Z., Procházka S.: Vývoj transgenních linií hrachu se zelpšenou rezistencí k virovým patogenům. Proceedings from XVIIth Czech and Slovak Plant Protection Conferen (cs)
  • Transgenosis approach in the development of pea lines with improved resistance to viral pathogens. Proceedings from XVIIth Czech and Slovak Plant Protection Conference, 12-14 December, Prague, pp. 116-120, 2006.ISBN: 80-213-1564-4
  • Transgenosis approach in the development of pea lines with improved resistance to viral pathogens. Proceedings from XVIIth Czech and Slovak Plant Protection Conference, 12-14 December, Prague, pp. 116-120, 2006.ISBN: 80-213-1564-4 (en)
skos:prefLabel
  • Švábová L., Griga M., Smýkal P., Hanáček P., Reinöhl V., Navrátil M., Šafářová D., Piáková Z., Procházka S.: Vývoj transgenních linií hrachu se zelpšenou rezistencí k virovým patogenům. Proceedings from XVIIth Czech and Slovak Plant Protection Conferen (cs)
  • Transgenosis approach in the development of pea lines with improved resistance to viral pathogens. Proceedings from XVIIth Czech and Slovak Plant Protection Conference, 12-14 December, Prague, pp. 116-120, 2006.ISBN: 80-213-1564-4
  • Transgenosis approach in the development of pea lines with improved resistance to viral pathogens. Proceedings from XVIIth Czech and Slovak Plant Protection Conference, 12-14 December, Prague, pp. 116-120, 2006.ISBN: 80-213-1564-4 (en)
skos:notation
  • RIV/48392952:_____/06:R0057318!RIV07-MZE-48392952
http://linked.open.../vavai/riv/strany
  • 116-120
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(QF3072), Z(MSM2678424601)
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 504212
http://linked.open...ai/riv/idVysledku
  • RIV/48392952:_____/06:R0057318
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Pea Enation Mosaic Virus (PEMV), Pea Seed-borne Mosaic Virus (PSbMV), coat protein gene, genetic modification, pathogen-derived resistance (PDR), post-transcriptional gene silencing (PTGS) (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [0ECB4CB5D480]
http://linked.open...v/mistoKonaniAkce
  • Praha
http://linked.open...i/riv/mistoVydani
  • Praha
http://linked.open...i/riv/nazevZdroje
  • Proceedings from XVIIth Czech and Slovak Plant Protection Conference
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...iv/tvurceVysledku
  • Griga, Miroslav
  • Hanáček, Pavel
  • Navrátil, Milan
  • Procházka, Stanislav
  • Reinöhl, Vilém
  • Smýkal, Petr
  • Šafářová, Dana
  • Švábová, Lenka
  • Piáková, Zuzana
http://linked.open...vavai/riv/typAkce
http://linked.open.../riv/zahajeniAkce
http://linked.open...n/vavai/riv/zamer
number of pages
http://purl.org/ne...btex#hasPublisher
  • Česká zemědělská univerzita v Praze. Katedra ochrany rostlin
https://schema.org/isbn
  • 80-213-1564-4
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