| Attributes | Values |
|---|
| rdf:type
| |
| rdfs:seeAlso
| |
| Description
| - The applicability of nanofibre material as a carrier for indigenous microbial communities was tested at sites contaminated by chlorinated ethenes (CE). CE are the second most common soil contaminants worldwide. One of the most successful remediation methods is enhanced reductive dehalogenation (ERD), which utilises anaerobic microbial degradation. However, cis1,2-DCE and cancerous vinyl chloride can unpredictably accumulate at some polluted sites during ERD. A deeper insight into the key-factors affecting the ERD process is therefore highly desirable. The nanofibre biomass carriers can help to characterize indigenous microbes in a well-defined and controlled way. The technique includes sample stabilisation for safe transport to a laboratory, isolation DNA and PCR/qPCR amplification. Nanofibre carriers of a high specific surface were installed in polluted underground water for at least two months. Stabilization of biomass on carriers was done four different ways, right after sampling: i) in cooling box, ii) using RNA later solution, iii) on dry ice and iv) in liquid nitrogen. DNA concentration was measured on Qubit (Life Technologies). Isolated DNA samples were analysed using PCR/qPCR for presence of certain bacterial taxa and vinyl chloride reductases. Nanofibre carriers ensured stable biomass growth and thus good DNA yield. The DNA yield was comparable for all stabilization procedures. All DNA samples were of high quality and able to be amplified by PCR/qPCR without inhibition. We were able to detect microbial taxa and VC reductases. Nanofibre biomass carriers are still being tested, the main issue is carrier selectivity. It has to be proved that microbial community in the groundwater and on the carrier does not differ.
- The applicability of nanofibre material as a carrier for indigenous microbial communities was tested at sites contaminated by chlorinated ethenes (CE). CE are the second most common soil contaminants worldwide. One of the most successful remediation methods is enhanced reductive dehalogenation (ERD), which utilises anaerobic microbial degradation. However, cis1,2-DCE and cancerous vinyl chloride can unpredictably accumulate at some polluted sites during ERD. A deeper insight into the key-factors affecting the ERD process is therefore highly desirable. The nanofibre biomass carriers can help to characterize indigenous microbes in a well-defined and controlled way. The technique includes sample stabilisation for safe transport to a laboratory, isolation DNA and PCR/qPCR amplification. Nanofibre carriers of a high specific surface were installed in polluted underground water for at least two months. Stabilization of biomass on carriers was done four different ways, right after sampling: i) in cooling box, ii) using RNA later solution, iii) on dry ice and iv) in liquid nitrogen. DNA concentration was measured on Qubit (Life Technologies). Isolated DNA samples were analysed using PCR/qPCR for presence of certain bacterial taxa and vinyl chloride reductases. Nanofibre carriers ensured stable biomass growth and thus good DNA yield. The DNA yield was comparable for all stabilization procedures. All DNA samples were of high quality and able to be amplified by PCR/qPCR without inhibition. We were able to detect microbial taxa and VC reductases. Nanofibre biomass carriers are still being tested, the main issue is carrier selectivity. It has to be proved that microbial community in the groundwater and on the carrier does not differ. (en)
|
| Title
| - Nanofibre Biomass Carriers as a Promising Tool for Genetic Analysis of Microbial Community from Highly Polluted Sites
- Nanofibre Biomass Carriers as a Promising Tool for Genetic Analysis of Microbial Community from Highly Polluted Sites (en)
|
| skos:prefLabel
| - Nanofibre Biomass Carriers as a Promising Tool for Genetic Analysis of Microbial Community from Highly Polluted Sites
- Nanofibre Biomass Carriers as a Promising Tool for Genetic Analysis of Microbial Community from Highly Polluted Sites (en)
|
| skos:notation
| - RIV/46747885:24620/14:#0000620!RIV15-MSM-24620___
|
| http://linked.open...avai/riv/aktivita
| |
| http://linked.open...avai/riv/aktivity
| |
| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
| |
| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
| |
| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/46747885:24620/14:#0000620
|
| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - nanofibre carriers; molecular genetic analysis (en)
|
| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...iv/tvurceVysledku
| |
| http://localhost/t...ganizacniJednotka
| |
![[RDF Data]](/fct/images/sw-rdf-blue.png)
![[cxml]](/fct/images/cxml_doc.png)
![[csv]](/fct/images/csv_doc.png)
![[text]](/fct/images/ntriples_doc.png)
![[turtle]](/fct/images/n3turtle_doc.png)
![[ld+json]](/fct/images/jsonld_doc.png)
![[rdf+json]](/fct/images/json_doc.png)
![[rdf+xml]](/fct/images/xml_doc.png)
![[atom+xml]](/fct/images/atom_doc.png)
![[html]](/fct/images/html_doc.png)