About: Atrazine-Based Self-Assembled Monolayers and Their Interaction with Anti-Atrazine Antibody: Building of an Immunosensor

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	As a part of our objective to build an immunosensor for the detection of the pesticide atrazine (ATZ) in environmental samples, we studied the self-assembling process of the disulfide derivative of the pesticide atrazine on a gold substrate. Atrazinebased self-assembled monolayers were characterized by ellipsometry, scanning tunneling microscopy, polarization modulation infrared reflection absorption spectroscopy (PM IRRAS), X-ray photoelectron spectroscopy and quartz crystal microbalance (QCM) measurements. Two different time constants for the adsorption process were observed, depending on the experimental method used. The QCM data reflect adsorption kinetics of the original disulfide compound, whereas ellipsometry and ex situ PM IRRAS refer to the formation of thiolate (ATZS) monolayers. In situ QCM data demonstrated the suitability of such monolayers for the detection of atrazine in aqueous samples. Exposure of the ATZS sensing surface to an anti atrazine antibody (anti-ATZ IgG) resulted in complete coverage of the surface by antibody, whereas approximately half of the antibody molecules were displaced from the QCM sensor surface by further addition of atrazine into the solution. As a part of our objective to build an immunosensor for the detection of the pesticide atrazine (ATZ) in environmental samples, we studied the self-assembling process of the disulfide derivative of the pesticide atrazine on a gold substrate. Atrazinebased self-assembled monolayers were characterized by ellipsometry, scanning tunneling microscopy, polarization modulation infrared reflection absorption spectroscopy (PM IRRAS), X-ray photoelectron spectroscopy and quartz crystal microbalance (QCM) measurements. Two different time constants for the adsorption process were observed, depending on the experimental method used. The QCM data reflect adsorption kinetics of the original disulfide compound, whereas ellipsometry and ex situ PM IRRAS refer to the formation of thiolate (ATZS) monolayers. In situ QCM data demonstrated the suitability of such monolayers for the detection of atrazine in aqueous samples. Exposure of the ATZS sensing surface to an anti atrazine antibody (anti-ATZ IgG) resulted in complete coverage of the surface by antibody, whereas approximately half of the antibody molecules were displaced from the QCM sensor surface by further addition of atrazine into the solution. (en)
Title	Atrazine-Based Self-Assembled Monolayers and Their Interaction with Anti-Atrazine Antibody: Building of an Immunosensor Atrazine-Based Self-Assembled Monolayers and Their Interaction with Anti-Atrazine Antibody: Building of an Immunosensor (en)
skos:prefLabel	Atrazine-Based Self-Assembled Monolayers and Their Interaction with Anti-Atrazine Antibody: Building of an Immunosensor Atrazine-Based Self-Assembled Monolayers and Their Interaction with Anti-Atrazine Antibody: Building of an Immunosensor (en)
skos:notation	RIV/44555601:13440/13:43886254!RIV15-MSM-13440___
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(GA13-19213S)
http://linked.open...iv/cisloPeriodika	52
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Hromadová, Magdaléna
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Jana Evangelisty Purkyně v Ústí nad Labem / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	62460
http://linked.open...ai/riv/idVysledku	RIV/44555601:13440/13:43886254
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	triazine herbicides; impedance spectroscopy; Quartz-crystal mickobalance (en)
http://linked.open.../riv/klicoveSlovo	impedance spectroscopy Quartz-crystal mickobalance triazine herbicides
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[8922E39DCBA0]
http://linked.open...i/riv/nazevZdroje	Langmuir
http://linked.open...in/vavai/riv/obor	CF
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Syntéza helikálních cyaninů, jejich chiralita, samouspořádání a redoxní přepínání
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	29
http://linked.open...iv/tvurceVysledku	Hof, Martin Hromadová, Magdaléna Pospíšil, Lubomír Sokolová, Romana Bulíčková, Jana Fischer-Durand, Natalie Salmain, Michele
http://linked.open...ain/vavai/riv/wos	000329332000006
issn	0743-7463
number of pages	9 (xsd:int)
http://bibframe.org/vocab/doi	10.1021/la404029j
http://localhost/t...ganizacniJednotka	13440

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