About: The effect of acetosyringone and L-cysteine cocultivation substances on genetic transformation efficiency in pea via combined biolistic and Agrobacterium-mediated methods.

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About: The effect of acetosyringone and L-cysteine cocultivation substances on genetic transformation efficiency in pea via combined biolistic and Agrobacterium-mediated methods. Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The combination of biolistics and Agrobacterium system allows taking advantage of both for effective genetic transformation systems (direct plus indirect) for various plant species. In our study we use the term agrolistic as the name for the method, which uses combination of (1) biolistics for mechanical disruption of explants, which follows (2) cocultivation in Agrobacterium suspension, thus proceeds the Agrobacterium-mediated transformation. Two chemical substances - acetosyringone (AS) and L-cysteine (CYS) were applied during cocultivation with the aim to enhance transformation efficacy. The combination of biolistics and Agrobacterium system allows taking advantage of both for effective genetic transformation systems (direct plus indirect) for various plant species. In our study we use the term agrolistic as the name for the method, which uses combination of (1) biolistics for mechanical disruption of explants, which follows (2) cocultivation in Agrobacterium suspension, thus proceeds the Agrobacterium-mediated transformation. Two chemical substances - acetosyringone (AS) and L-cysteine (CYS) were applied during cocultivation with the aim to enhance transformation efficacy. (en) Kombinace biolistické a agrobakteriální metody transformace umožňuje využít výhody obou transformačních systémů (přímého i nepřímého) pro různé rostlinné druhy. Termín agrolistika označuje metodu, která využívá biolistiku pro mechanické narušení explantátů a následnou kokultivaci v agrobakteriální suspenzi. Byl odzkoušen vliv acetosyringonu a L-cysteinu s cílem zvýšit efektivitu transformace. (cs)
Title	The effect of acetosyringone and L-cysteine cocultivation substances on genetic transformation efficiency in pea via combined biolistic and Agrobacterium-mediated methods. Vliv kokultivačních látek acetosyringonu a L-cysteinu na efektivitu transformace u hrachu (Pisum sativum L.) kombinovanou biolistickou a agrobakteriální metodou. (cs) The effect of acetosyringone and L-cysteine cocultivation substances on genetic transformation efficiency in pea via combined biolistic and Agrobacterium-mediated methods. (en)
skos:prefLabel	The effect of acetosyringone and L-cysteine cocultivation substances on genetic transformation efficiency in pea via combined biolistic and Agrobacterium-mediated methods. Vliv kokultivačních látek acetosyringonu a L-cysteinu na efektivitu transformace u hrachu (Pisum sativum L.) kombinovanou biolistickou a agrobakteriální metodou. (cs) The effect of acetosyringone and L-cysteine cocultivation substances on genetic transformation efficiency in pea via combined biolistic and Agrobacterium-mediated methods. (en)
skos:notation	RIV/26784246:_____/08:#0000109!RIV09-MSM-26784246
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(1M06030), Z(MSM2678424601)
http://linked.open...vai/riv/dodaniDat	2009
http://linked.open...aciTvurceVysledku	Griga, Miroslav Švábová, Lenka
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Agritec Plant Research s.r.o.
http://linked.open...dnocenehoVysledku	364986
http://linked.open...ai/riv/idVysledku	RIV/26784246:_____/08:#0000109
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	acetosyringone, cysteine, biolistic, genetic transformation, pea (en)
http://linked.open.../riv/klicoveSlovo	pea cysteine acetosyringone biolistic genetic transformation
http://linked.open...ontrolniKodProRIV	[E6D860C34BCC]
http://linked.open...v/mistoKonaniAkce	Křtiny, 9.-10.9.2008
http://linked.open...i/riv/mistoVydani	MZLU Brno, Česká republika
http://linked.open...i/riv/nazevZdroje	Funkční genomika a proteomika ve šlechtění rostlin.
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Functional genomics and proteomics for crop improvement
http://linked.open...UplatneniVysledku	2008
http://linked.open...iv/tvurceVysledku	Griga, Miroslav Švábová, Lenka Ohnoutková, Ludmila
http://linked.open...vavai/riv/typAkce	CST - Celostátní
http://linked.open.../riv/zahajeniAkce	2008-01-01 (xsd:date)
http://linked.open...n/vavai/riv/zamer	Study and utilization of biotechnological and molecular methods in genetic and breeding research of genus Pisum and Linum
number of pages	6 (xsd:int)
http://purl.org/ne...btex#hasPublisher	MZLU Brno, Česká republika
https://schema.org/isbn	978-80-7375-234-7
is http://linked.open...avai/riv/vysledek of	The effect of acetosyringone and L-cysteine cocultivation substances on genetic transformation efficiency in pea via combined biolistic and Agrobacterium-mediated methods. The effect of acetosyringone and L-cysteine cocultivation substances on genetic transformation efficiency in pea via combined biolistic and Agrobacterium-mediated methods. The effect of acetosyringone and L-cysteine cocultivation substances on genetic transformation efficiency in pea via combined biolistic and Agrobacterium-mediated methods. The effect of acetosyringone and L-cysteine cocultivation substances on genetic transformation efficiency in pea via combined biolistic and Agrobacterium-mediated methods.

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