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| Description
| - We have used several molecular methods to assess the genetic stability of long-term propagated (24 years) multiple shoot in vitro culture of pea (Pisum sativum L.). We focused on assessing the stability of repetitive sequences, such as simple sequence repeats (SSR) and retrotransposons, both comprising a large part of genome. No differences were found between controls and long-term or newly established in vitro (one subculture cycle) samples were investigated by the SSR, inter-repeats (ISSR) or inter-retrotransposon amplified polymorphism (IRAP) method. However, the more global amplified fragment length polymorphism (AFLP) and the methylation sensitive MSAP methods detected 11 and 18% polymorphism. Some evidence of retrotransposon re-arrangement was observed by sequence-specific amplification polymorphism. Based on these results, multiple shoot culture of pea maintained over a long period may be considered as a true to type multiplication method of the original genotype.
- We have used several molecular methods to assess the genetic stability of long-term propagated (24 years) multiple shoot in vitro culture of pea (Pisum sativum L.). We focused on assessing the stability of repetitive sequences, such as simple sequence repeats (SSR) and retrotransposons, both comprising a large part of genome. No differences were found between controls and long-term or newly established in vitro (one subculture cycle) samples were investigated by the SSR, inter-repeats (ISSR) or inter-retrotransposon amplified polymorphism (IRAP) method. However, the more global amplified fragment length polymorphism (AFLP) and the methylation sensitive MSAP methods detected 11 and 18% polymorphism. Some evidence of retrotransposon re-arrangement was observed by sequence-specific amplification polymorphism. Based on these results, multiple shoot culture of pea maintained over a long period may be considered as a true to type multiplication method of the original genotype. (en)
- Pomocí molekulárních metod byla studována genetické a epigenetická stabilita dlouhodobé in vitro kultury vzrostných prýtů hrachu. Mikrosatelitními a retrotransposonovými markery nebyla zjištěny žádné významné změny. Tyto byly detekovány pouze pomocí metylačně citlivé analýzy (MSAP), kdy byl zjištěn 11 a 18% polymorfismus. Tyto změny v metylaci genomové DNA byly potvrzeny pomocí měření celkového zastoupení metyl-cytosinu metdou HPCE. (cs)
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| Title
| - Assessment of genetic and epigenetic stability in long-term in vitro shoot culture of pea (Pisum sativum L.)
- Assessment of genetic and epigenetic stability in long-term in vitro shoot culture of pea (Pisum sativum L.) (en)
- Studium genetické a epigenetické stability dlouhodobé in vitro kultury hrachu (Pisum sativum L.) (cs)
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| skos:prefLabel
| - Assessment of genetic and epigenetic stability in long-term in vitro shoot culture of pea (Pisum sativum L.)
- Assessment of genetic and epigenetic stability in long-term in vitro shoot culture of pea (Pisum sativum L.) (en)
- Studium genetické a epigenetické stability dlouhodobé in vitro kultury hrachu (Pisum sativum L.) (cs)
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| skos:notation
| - RIV/26784246:_____/07:#0000045!RIV08-MSM-26784246
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/26784246:_____/07:#0000045
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - Clonal propagation, Genetic stability, DNA methylation, Pisum sativum, Retrotransposons, Somaclonal variation (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
| - DE - Spolková republika Německo
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Griga, Miroslav
- Smýkal, Petr
- Valledor, Luis
- Rodriguzez, Roberto
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| http://linked.open...n/vavai/riv/zamer
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| is http://linked.open...avai/riv/vysledek
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