About: ASSESSMENT OF INDOXYLACETATE AS A SUBSTRATE FOR ACETYLCHOLINESTERASE

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Biochemical assessment of acetylcholinesterase (AChE) activity is a standard protocol in biochemistry, pharmacology, and toxicology. In 1960s, Ellman´s method was developed for the purpose of AChE activity assay and it became routine after some optimizations. The method has however some disadvantages and limitations in its performance. Firstly, the used reagents are labile and fresh reagents have to be prepared at least every day. Secondly, some compounds interfere in the assay. Glutathione, thiol containing compuonds and oxime containing drugs are the most relevant. We infer to introduce indoxylacetate based assay for assessment of AChE activity. The assay was tested and basic parameters were established for the indoxylacetate. Michaelis constant and maximum reaction velocity were calculated for electric eel AChE. The assay was also performed for estimation of oxime rectivators obidoxime, pralidoxime, and HI-6 efficacy after paraoxon ethyl inhibition. The whole experiment was repeated using Ellman´s method and the both methods were compared. Significant advantages of indoxylacetate as a substrate are reported. Biochemical assessment of acetylcholinesterase (AChE) activity is a standard protocol in biochemistry, pharmacology, and toxicology. In 1960s, Ellman´s method was developed for the purpose of AChE activity assay and it became routine after some optimizations. The method has however some disadvantages and limitations in its performance. Firstly, the used reagents are labile and fresh reagents have to be prepared at least every day. Secondly, some compounds interfere in the assay. Glutathione, thiol containing compuonds and oxime containing drugs are the most relevant. We infer to introduce indoxylacetate based assay for assessment of AChE activity. The assay was tested and basic parameters were established for the indoxylacetate. Michaelis constant and maximum reaction velocity were calculated for electric eel AChE. The assay was also performed for estimation of oxime rectivators obidoxime, pralidoxime, and HI-6 efficacy after paraoxon ethyl inhibition. The whole experiment was repeated using Ellman´s method and the both methods were compared. Significant advantages of indoxylacetate as a substrate are reported. (en)
Title	ASSESSMENT OF INDOXYLACETATE AS A SUBSTRATE FOR ACETYLCHOLINESTERASE ASSESSMENT OF INDOXYLACETATE AS A SUBSTRATE FOR ACETYLCHOLINESTERASE (en)
skos:prefLabel	ASSESSMENT OF INDOXYLACETATE AS A SUBSTRATE FOR ACETYLCHOLINESTERASE ASSESSMENT OF INDOXYLACETATE AS A SUBSTRATE FOR ACETYLCHOLINESTERASE (en)
skos:notation	RIV/25656180:_____/11:#0000014!RIV13-MPO-25656180
http://linked.open...avai/predkladatel	VAKOS XT a. s.
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(FR-TI1/541)
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Povrazník, Ing., Jozef Hrabinová, Martina Pohanka, Doc. RNDr. PhD, Miroslav
http://linked.open.../riv/druhVysledku	O - Ostatní výsledky nezařaditelné do žádného z výše uvedených druhů výsledku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	VAKOS XT a.s.
http://linked.open...dnocenehoVysledku	187268
http://linked.open...ai/riv/idVysledku	RIV/25656180:_____/11:#0000014
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	acetylcholinesterase, Ellman´s method, oxime rectivators, obidoxime, pralidoxime, HI-6 (en)
http://linked.open.../riv/klicoveSlovo	Ellman´s method obidoxime oxime rectivators pralidoxime HI-6 acetylcholinesterase
http://linked.open...ontrolniKodProRIV	[2A6B4E024EF8]
http://linked.open...in/vavai/riv/obor	KA
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	*Construction of simple dipstickfor organophosphates assay
http://linked.open...UplatneniVysledku	2011
http://linked.open...iv/tvurceVysledku	Hrabinová, Martina Pohanka, Doc. RNDr. PhD, Miroslav Povrazník, Ing., Jozef

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